[1]李金平,刘尧邦,刘奇伦.超氧化物歧化酶2在乳腺癌中的表达及其临床意义[J].南方医科大学学报,2020,(08):1103-1111.
 Expression of superoxide dismutase 2 in breast cancer and its clinical significance[J].Journal of Southern Medical University,2020,(08):1103-1111.
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超氧化物歧化酶2在乳腺癌中的表达及其临床意义()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2020年08期
页码:
1103-1111
栏目:
出版日期:
2020-07-30

文章信息/Info

Title:
Expression of superoxide dismutase 2 in breast cancer and its clinical significance
作者:
李金平刘尧邦刘奇伦
关键词:
乳腺癌癌症基因组图谱超氧化物歧化酶2生物信息学分析
Keywords:
breastcancer TCGA superoxide dismutase 2 bioinformatic analysis
文献标志码:
A
摘要:
目的 评估超氧化物歧化酶2(SOD2)在乳腺癌中表达及预后价值,揭示其在乳腺癌发生发展中可能的作用机制。方法 采 用R 软件对TCGA数据进行生信分析,用Wilcoxon秩和检验和Wilcoxon符号秩和检验分析SOD2在乳腺癌中的表达及临床相 关性,应用KEGG的基因集进行基因富集分析(GSEA),然后通过STRING数据库构建蛋白质相互作用网络,用Cytoscape 软件进行关键基因筛选,两基因相关性使用Pearson’s相关性分析。通过免疫组化和 RT-qPCR 法对2018~2019年收治的原发性乳腺癌组织、癌旁组织样本各60例进行临床验证。结果 SOD2在乳腺癌组织中的表达明显低于乳腺正常组织,其表达与乳腺癌TNM分期和腋窝淋巴结转移显著相关(P<0.05)。Kaplan-Meier法生存分析显示SOD2高表达患者的无复发生存、无远处转移生存和后进展生存期均显著低于SOD2低表达者(P<0.05);GSEA富集分析结果显示SOD2在JAK-STAT信号通路中发挥重要的生物学作用。构建PPI网络筛选出关键基因IL10和STAT4,二者均与SOD2呈正相关性。SOD2在临床乳腺癌组织样本中高表达,其表达与腋窝淋巴结有无转移、雌激素受体表达及雄激素受体表达有显著性差异(P<0.05)。结论 SOD2在乳腺癌中的表达与TNM分期、腋窝淋巴结转移显著相关,SOD2可能通过调控IL10和/或STAT4介导JAK/STAT信号通路影响乳腺癌细胞的增殖、侵袭和转移。
Abstract:
Objective To evaluate the expression and prognostic value of superoxide dismutase 2 (SOD2) in breast cancer and explore its possible role in the occurrence and progression of breast cancer. Methods We performed bioinformatics analysis of the TCGA data for the expression and clinical relevance of SOD2 in patients with breast cancer. Gene enrichment analysis (GSEA) was performed using the KEGG gene set, the protein interaction network was constructed using the STRING database, and the key genes were screened using Cytoscape software. We also collected 60 pairs of primary breast cancer tissue samples and adjacent samples for detecting SOD2 expressions using immunohistochemistry and RT-qPCR and analyzed the correlation of SOD2 expression with the clinicopathological parameters of the patients. Results The expression of SOD2 was significantly lower in breast cancer tissue than in adjacent tissues with significant correlation with TNM stage and axillary lymph node metastasis (P<0.05). Kaplan-Meier survival analysis showed that the recurrence-free survival, distant metastasis-free survival (RFS) and post-progressive survival were significantly shorted in patients with high SOD2 expression than in those with low SOD2 expression (P<0.05). GSEA enrichment analysis indicated that SOD2 played an important role in the JAK-STAT signaling pathway. IL10 and STAT4 were identified as the key genes in the PPI network, and they were both positively correlated with SOD2. In the 60 pairs of clinical samples, SOD2 was highly expressed in breast cancer tissues with close correlation with axillary lymph node metastasis and the expressions of estrogen receptor and androgen receptor (P<0.05). Conclusion The expression of SOD2 in breast cancer is significantly correlated with TNM stage and axillary lymph node metastasis. SOD2 may affect the proliferation, invasion and metastasis of breast cancer cells possibly by regulating IL10 and/or STAT4 to affect the JAK/STAT signaling pathway.

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更新日期/Last Update: 2020-07-29