[1]肖 斌,周 泉,雷 婷,等.SARS-Cov-2实验室检测技术的应用及展望[J].南方医科大学学报,2020,(04):601-605.[doi:10.12122/j.issn.1673-4254.2020.04.24]
 Application of laboratory diagnostic technologies for SARS-CoV-2: current progress andprospect[J].Journal of Southern Medical University,2020,(04):601-605.[doi:10.12122/j.issn.1673-4254.2020.04.24]
点击复制

SARS-Cov-2实验室检测技术的应用及展望()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2020年04期
页码:
601-605
栏目:
出版日期:
2020-04-30

文章信息/Info

Title:
Application of laboratory diagnostic technologies for SARS-CoV-2: current progress and prospect
作者:
肖 斌周 泉雷 婷何咏茵李林海
关键词:
新型冠状病毒qRT-PCR抗体数字PCRSHERLOCK
Keywords:
SARS-Cov-2 qRT-PCR antibody digital PCR SHERLOCK
DOI:
10.12122/j.issn.1673-4254.2020.04.24
文献标志码:
A
摘要:
新型冠状病毒肺炎疫情爆发以来,我国的病毒检测能力不断提高,检测技术也在不断地发展和创新。以实时荧光定量PCR(qRT-PCR)和宏基因组测序为代表的病毒检测技术在辅助疾病确诊、监测病毒变异等方面发挥了关键作用;基于血清/血浆IgM/IgG抗体的检测技术是辅助确诊的重要方法;高特异性的逆转录环介导等温扩增技术、能够核酸定量的数字PCR和灵敏度更高的SHERLOCK技术也极具特色与发展潜力。以上检测技术代表了病原微生物检测技术的现在及未来发展方向。本文对新型冠状病毒检测技术的基本原理、技术特点及应用现状进行探讨。
Abstract:
Since the outbreak of COVID-19 pandemic, the detection capability has been improving and the detection techniques have been evolving with innovations. qRT- PCR and mNGS, which represent the current mainstay diagnostic technologies, play key roles in disease diagnosis and monitoring of virus variation. The detection technologies based on serum and plasma IgM and IgG antibodies are important for auxiliary diagnosis. RT-LAMP is highly specific for a diagnostic purpose. Digital PCR could quantitatively detect nucleic acid and SHERLOCK has a higher sensitivity. These techniques all have great potential for future development and application for pathogen detection. In this review the authors summarize the basic rationales, technical characteristics and the current application of the SARS-CoV-2 detection techniques.

相似文献/References:

[1]傅晓彬,李杰生,黄 洲,等.蒲公英果实征: 9例新型冠状病毒肺炎的CT征象[J].南方医科大学学报,2020,(02):159.[doi:10.12122/j.issn.1673-4254.2020.02.06]
 Dandelion clock-like sign on CT for diagnose of COVID-19[J].Journal of Southern Medical University,2020,(04):159.[doi:10.12122/j.issn.1673-4254.2020.02.06]
[2]邱 峰,王慧君,张子康,等.新型冠状病毒SARS-CoV-2的实验室检测技术[J].南方医科大学学报,2020,(02):164.[doi:10.12122/j.issn.1673-4254.2020.02.16]
 Laboratory testing techniques for SARS-CoV-2[J].Journal of Southern Medical University,2020,(04):164.[doi:10.12122/j.issn.1673-4254.2020.02.16]
[3]杭建峰,孙朝晖,李林海.临床实验室检测新型冠状病毒感染防控预案的制定及疫情后时代应对策略的思考[J].南方医科大学学报,2020,(04):606.[doi:10.12122/j.issn.1673-4254.2020.04.25]
 Prevention and control of SARS-CoV-2 infection in clinical laboratories: implementationof contingency plan and post-pandemic response strategies[J].Journal of Southern Medical University,2020,(04):606.[doi:10.12122/j.issn.1673-4254.2020.04.25]

更新日期/Last Update: 2020-04-30