[1]汤凯淇,张 瑜,陈丽竹,等.和厚朴酚对人舌癌CAL-27细胞增殖、迁移及凋亡的影响[J].南方医科大学学报,2020,(04):580-585.[doi:10.12122/j.issn.1673-4254.2020.04.21]
 Effect of honokiol on proliferation, migration and apoptosis of human tongue cancerCAL-27 cells in vitro[J].Journal of Southern Medical University,2020,(04):580-585.[doi:10.12122/j.issn.1673-4254.2020.04.21]
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和厚朴酚对人舌癌CAL-27细胞增殖、迁移及凋亡的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2020年04期
页码:
580-585
栏目:
出版日期:
2020-04-30

文章信息/Info

Title:
Effect of honokiol on proliferation, migration and apoptosis of human tongue cancer CAL-27 cells in vitro
作者:
汤凯淇张 瑜陈丽竹屈 直
关键词:
和厚朴酚CAL-27细胞增殖迁移凋亡
Keywords:
honokiol CAL-27 cells proliferation migration apoptosis
DOI:
10.12122/j.issn.1673-4254.2020.04.21
文献标志码:
A
摘要:
目的 探讨和厚朴酚(HNK)对人舌鳞癌CAL-27细胞增殖、迁移和凋亡的影响。方法 采用含10%胎牛血清的DMEM培养基培养CAL-27细胞。并将其分为对照组和3个实验组,实验组分别加入20、40、60 μmol/L的HNK进行处理。用MTT法检测 不同浓度和厚朴酚对CAL-27细胞增殖的影响;划痕实验观察CAL-27细胞迁移能力;Hoechst33342荧光染色法和Annexin VFITC/PI法检测CAL-27细胞凋亡数及凋亡率;Western blot检测CAL-27细胞内p-Pi3k、p-Fak、Fak、MMP2、MMP9、p-Akt、Akt、Bax、Bcl-2和Cleaved-Caspase-3的蛋白表达量。结果 HNK(0、20、40、60 μmol/L)处理24 h后CAL-27细胞增殖、迁移能力减弱;且随HNK浓度增大细胞凋亡数升高,凋亡率分别为(6.53±1.80)%、(15.24±2.06)%、(35.03±2.42)%、(48.13±4.61)%,细胞内p-Pi3k、p-Fak、p-Akt、MMP2、MMP9和Bcl-2蛋白表达量减少而Bax、Cleaved-Caspase-3蛋白表达量增加(P<0.01)。结论 HNK能抑制CAL-27细胞体外增殖及迁移,同时还能诱导其凋亡,作用机制可能与调控细胞内p-Pi3k、p-Fak、p-Akt、MMP2、MMP9、Bax、Bcl-2和Cleaved-Caspase-3的蛋白表达量有关。
Abstract:
Objective To study the effects of honokiol on proliferation, migration and apoptosis of human tongue carcinoma CAL-27 cells. Methods Routinely cultured CAL-27 cells were treated with 20, 40, or 60 μmol/L honokiol and the changes in cell proliferation were assessed with MTT assay. The scratch wound healing assay was used to assess the migration ability of the treated cells, and the cell apoptosis was detected with Hoechst33342 fluorescence staining and annexin V-FITC/PI method. The protein expression levels of p-Pi3k, p-Fak, Fak, MMP-2, MMP-9, p-Akt, Akt, Bax, Bcl-2 and cleaved-caspase-3 in the treated cells were detected using Western blotting. Results Treatment with honokiol at 20, 40, and 60 μmol/L for 24 h significantly lowered the proliferation and migration ability of CAL-27 cells. The number of apoptotic cells increased with the increase of honokiol concentration, which resulted in a cell apoptosis rate of (15.24±2.06)% at 20 μmol/L, (35.03±2.42)% at 40 μmol/L, and (48.13±4.61)% at 60 μmol/L, as compared with (6.53±1.80)% in the control group. The expressions of p-Pi3k, p-Fak, MMP-2, MMP-9, p-Akt and BCL-2 decreased and those of Bax and cleaved-caspase-3 increased significantly in the cells after the treatment (P<0.01). Conclusion Honokiol can inhibit the proliferation and migration and induce apoptosis of CAL-27 cells in vitro possibly by regulating the expressions of p-Pi3k, p-Fak, MMP-2, MMP-9, p-Akt, Bax, Bcl-2 and cleaved-caspase-3.
更新日期/Last Update: 2020-04-30