[1]纪雪霞,郭远波,邱倩琪,等.丙泊酚抑制内毒素血症中巨噬细胞发生焦亡的分子机制[J].南方医科大学学报,2020,(04):525-530.[doi:10.12122/j.issn.1673-4254.2020.04.12]
 Molecular mechanism underlying the inhibitory effect of propofol on lipopolysaccharideinduced pyroptosis of mouse bone marrow-derived macrophages[J].Journal of Southern Medical University,2020,(04):525-530.[doi:10.12122/j.issn.1673-4254.2020.04.12]
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丙泊酚抑制内毒素血症中巨噬细胞发生焦亡的分子机制()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2020年04期
页码:
525-530
栏目:
出版日期:
2020-04-30

文章信息/Info

Title:
Molecular mechanism underlying the inhibitory effect of propofol on lipopolysaccharideinduced pyroptosis of mouse bone marrow-derived macrophages
作者:
纪雪霞郭远波邱倩琪王志鹏王 研吉锦泉孙 强蔡宇晶周国斌
关键词:
内毒素血症异丙酚焦亡巨噬细胞
Keywords:
endotoxemia propofol pyrotopsis macrophages
DOI:
10.12122/j.issn.1673-4254.2020.04.12
文献标志码:
A
摘要:
目的 探讨丙泊酚抑制巨噬细胞发生焦亡的分子机制。方法 提取小鼠骨髓来源巨噬细胞,分为对照组、脂多糖(LPS)+三磷酸腺苷(ATP)刺激组以及丙泊酚+LPS+ATP组。对照组不给予任何处理;LPS+ATP组先给予脂多糖(LPS) 1 μg/mL刺激4 h,再加三磷酸腺苷(ATP)4 mmol/L刺激1 h;丙泊酚+LPS+ATP组先同时给予50 μmmol/L丙泊酚+LPS 1 μg/mL刺激4 h,再加ATP刺激1 h。处理完毕则收集细胞培养上清液和细胞。分别通过CCK8、流式分析检测细胞活力,酶联免疫吸附测定法法检测细胞上清炎症因子IL-1β、IL-18含量;用免疫印迹检测细胞caspase-1蛋白表达及细胞膜Toll样受体-4(TLR4)表达;流式分析及免疫组化荧光检测细胞焦亡情况。结果 LPS+ATP可导致小鼠骨髓来源巨噬细胞(BMDM)活力显著降低(P<0.05);炎症因子IL-1β、IL-18升高(P<0.05);活化的caspase-1蛋白增加,细胞膜表面TLR-4表达升高(P<0.05);予丙泊酚处理后,可改善LPS+ATP诱导的上述指标变化。结论 LPS+ATP可诱导BMDM发生焦亡,丙泊酚有效抑制这种细胞死亡,提示丙泊酚麻醉有益于临床脓毒症患者的手术,有效调节患者免疫状态。
Abstract:
Objective To investigate the molecular mechanism underlying the inhibitory effect of propofol on pyroptosis of macrophages. Methods Macrophages derived from bone marrow were extracted and divided into three groups: control group, LPS+ATP group and propofol+LPS+ATP group. The control group was not given any treatment; LPS+ATP group was given LPS 1 μg/mL stimulation for 4 h, then ATP 4 mM stimulation for 1 h; Propofol+LPS+ATP group was given propofol+LPS 1 μg/mL stimulation for 4 h, then ATP stimulation for 1 h. After treatment, the supernatant and cells of cell culture were collected. the cell activity was detected by CCK8 and flow cytometry. The inflammatory cytokines IL-1βand IL-18 were detected by Elisa. Western blot was used to detect the expression of caspase-1 protein and TLR4 on cell membran Immunohistochemical fluorescence was used to detect apoptosis of cells. Results LPS+ATP significantly decreased the viability of the macrophages and increased the cellular production of IL-1β and IL-18, activation of caspase-1 protein and the expression of TLR-4 on the cell membrane (P<0.05). Treatment with propofol obviously reversed the changes induced by LPS+ATP. Conclusion LPS+ATP can induce pyroptosis of mouse bone marrow-derived macrophages, and propofol effectively inhibits such cell death, suggesting that propofol anesthesia is beneficial during operation and helps to regulate the immune function of in patients with sepsis.

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更新日期/Last Update: 2020-04-30