[1]李 婷,邓树豪,雷 雯,等.氢气水通过升高Nrf2的表达减轻百草枯诱导的肺纤维化[J].南方医科大学学报,2020,(02):233-239.[doi:10.12122/j.issn.1673-4254.2020.02.10]
 Hydrogen water alleviates paraquat-induced lung fibroblast injury in vitro by enhancingNrf2 expression[J].Journal of Southern Medical University,2020,(02):233-239.[doi:10.12122/j.issn.1673-4254.2020.02.10]
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氢气水通过升高Nrf2的表达减轻百草枯诱导的肺纤维化()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2020年02期
页码:
233-239
栏目:
出版日期:
2020-02-29

文章信息/Info

Title:
Hydrogen water alleviates paraquat-induced lung fibroblast injury in vitro by enhancing Nrf2 expression
作者:
李 婷邓树豪雷 雯李振坤吴文娟张 涛董昭兴
关键词:
肺成纤维细胞百草枯氢气水核转录相关因子2
Keywords:
lung fibroblasts paraquat hydrogen water Nrf2
DOI:
10.12122/j.issn.1673-4254.2020.02.10
文献标志码:
A
摘要:
目的 探讨氢气水依赖Nrf2对百草枯诱导的肺成纤维细胞增殖,转化及胶原分泌的影响。方法 人肺成纤维细胞(HFL-1)体外培养,采用RNA干扰技术沉默HLC-1细胞中Nrf2的表达,设置对照组、百草枯(PQ)染毒组、PQ+氢气水治疗组、PQ+氢气水+Nrf2siRNA综合干预组。设置对照组:人肺成纤维细胞(HFL1)正常培养;染毒组:HFL1+600 μmol/L PQ 24 h;氢气水治疗组:HFL1+600 μmol/L PQ24 h后+氢气水;综合干预治疗组:HFL1转染siRNA+600 μmol/LPQ 24 h后+氢气水。MTT法检测细胞的增殖,Western blot、qt-PCR、免疫荧光检测各组肺成纤维细胞Col-Ⅰ、Col-Ⅲ、α-SMA、Nrf2的表达,同时ELISA检测抗氧化物SOD、CAT、GSH的含量。结果 氢气水治疗组较PQ染毒组Col-Ⅰ、Col-Ⅲ、α-SMA的表达明显降低,干扰Nrf2基因表达后,氢气水疗效较治疗组显著下降(P<0.05)。氢气水治疗组Nrf2表达量较PQ染毒组增加(P<0.05)。结论 氢气水可能通过诱导Nrf2的表达促进肺成纤维细胞的增殖和抗氧化物的生成,同时抑制其转化和分泌功能。
Abstract:
Objective To investigate the effects of hydrogen water on proliferation, differentiation, collagen secretion and Nrf2 expression in paraquat-induced human lung fibroblasts. Methods In vitro cultured human lung fibroblasts (HFL1) exposed to 600 μmol/L paraquat (PQ) for 24 h were treated with hydrogen water with or without RNA interference of Nrf2 expression. The changes in the cell proliferation were examined using MTT assay, and the expressions of Col-I, Col-III, α-SMA and Nrf2 in the cells were detected using Western blotting, real-time quantitative PCR and immunofluorescence assay. The contents of SOD, CAT and GSH in the cells were determined with ELISA. Results Compared with the PQ-exposed cells, the cells with hydrogen water treatment showed significantly lowered expressions of Col-I, Col-III, and α-SMA. Interference of Nrf2 expression obviously attenuated the effect of hydrogen water on PQ-exposed cells. Hydrogen water treatment significantly increased the expression of Nrf2 and promoted the production of the antioxidants in PQ-exposed lung fibroblasts. Conclusion Hydrogen water enhances Nrf2 expression to promote the proliferation and production of antioxidants and inhibit the differentiation and collagen secretion in PQ-exposed human lung fibroblasts in vitro.

相似文献/References:

[1]董昭兴,康庆鑫,雷雯,等.IL-17对肺成纤维细胞的增殖、转化和胶原合成作用[J].南方医科大学学报,2012,(01):75.
[2]董昭兴,邰文琳,雷雯,等.IL-27及其受体对肺成纤维细胞增殖及转化的影响[J].南方医科大学学报,2015,(10):1411.
[3]彭志允,常平,王华,等.高强度血液灌流联合长时程血液滤过在百草枯中毒救治中的应用:1例报告[J].南方医科大学学报,2015,(10):1515.

更新日期/Last Update: 2020-03-14