[1]古文清,潘文斌,朱 茜,等.KIF3A在单侧输尿管梗阻小鼠肾脏中的表达及意义[J].南方医科大学学报,2020,(02):219-224.[doi:10.12122/j.issn.1673-4254.2020.02.07]
 Expression of kinesin KIF3A in the kidney of mice with unilateral ureteral obstruction[J].Journal of Southern Medical University,2020,(02):219-224.[doi:10.12122/j.issn.1673-4254.2020.02.07]
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KIF3A在单侧输尿管梗阻小鼠肾脏中的表达及意义()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2020年02期
页码:
219-224
栏目:
出版日期:
2020-02-29

文章信息/Info

Title:
Expression of kinesin KIF3A in the kidney of mice with unilateral ureteral obstruction
作者:
古文清潘文斌朱 茜肖 潇赵岩岩刘雅琴刘 俊李 明
关键词:
KIF3A肾间质纤维化wnt/β-catenin信号通路EMT
Keywords:
KIF3A renal tubulointerstitial fibrosis wnt4/β-catenin signaling pathway epithelial-mesenchymal transition
DOI:
10.12122/j.issn.1673-4254.2020.02.07
文献标志码:
A
摘要:
目的 通过研究KIF3A在单侧输尿管梗阻(UUO)小鼠和TGF-β1诱导的NRK-52E细胞中的表达情况,探讨KIF3A在肾间质纤维化中的作用。方法 将36只C57BL/6J小鼠随机分为假手术组(Sham, n=18)和单侧输尿管梗阻组(UUO, n=18),分别于术后7、14、21 d 处死小鼠。苏木素-伊红(HE)染色、马松(Masson)三色法染色和天狼星红(Sirius Red)染色观察小鼠肾间质纤维化程度,RT-PCR、免疫组织化学(IHC)和免疫印迹(Western blot)检测小鼠肾组织KIF3A的表达和分布。免疫印迹检测小鼠肾组织KIF3A、E-cadherin 和α-SMA 蛋白的表达。TGF-β1诱导NRK-52E细胞转分化后,免疫印迹检测KIF3A、E-cadherin 、α-SMA、Wnt4和β-catenin 蛋白的表达。结果 与Sham组相比,UUO小鼠肾间质纤维化程度随着梗阻时间延长而增加,表明造模成功。同时,KIF3A mRNA和蛋白表达均增高,于21 d达到高峰,α-SMA蛋白表达显著上调,E-cadherin蛋白表达明显降低。NRK-52E细胞发生转分化时,KIF3A蛋白的表达增多(P<0.001),同时wnt/β-catenin信号通路中Wnt4和β-catenin蛋白表达增加(P<0.05,P<0.0001)。结论 KIF3A可能通过wnt/β-catenin信号通路介导的上皮细胞-间充质转化参与肾纤维化的发生发展过程。
Abstract:
Objective To investigate the expression of KIF3A in mice with unilateral ureteral obstruction (UUO) and TGF-β1- induced NRK-52E cells and the role of KIF3A in renal tubular epithelial cell transdifferentiation. Methods Thirty-six C57BL/6J mice were randomly divided into the sham group (n=18) and UUO group (n=18). Six mice in each group were sacrificed at 7, 14 and 21days after the operation. The degree of renal tubulointerstitial fibrosis of the mice was observed by HE staining, Masson trichrome staining and Sirius red staining. The expression and distribution of KIF3A in the kidney of the mice was detected using RT-PCR, Western blotting and immunohistochemistry. Western blotting was used to detect the expression of KIF3A, E-cadherin and α-SMA proteins in the renal tissue of the mice. The expressions of KIF3A, E-cadherin, α-SMA, Wnt4 and β-catenin proteins in NRK-52E cells with TGF-β1-induced transdifferentiation were detected by Western blotting. Results Compared with the sham-operated mice, the mice with UUO showed worsened renal interstitial fibrosis with the increase of obstruction time, indicating successful modeling. The expressions of KIF3A mRNA and protein increased progressively and reached the peaked level at 21 days after UUO. The expression of α-SMA protein was significantly increased while E-cadherin protein expression was significantly reduced after UUO. The transdifferentiated NRK-52E cells showed significantly increased expressions of KIF3A (P<0.001), Wnt4 (P<0.05) and β-catenin proteins (P<0.0001). Conclusions KIF3A may participate in the development of renal fibrosis through epithelial-mesenchymal transition mediated by wnt/β-catenin signaling pathway.

相似文献/References:

[1]黄玉宇,徐安平,周姗姗,等.不同剂量氯沙坦对大鼠单侧输尿管梗阻模型MCP1和TGF-β1的影响[J].南方医科大学学报,2011,(08):1405.
[2]肖小燕,莫立乾,宋少练,等.复方黄甘延缓5/6肾切除大鼠慢性肾功能衰竭[J].南方医科大学学报,2014,(11):1661.

更新日期/Last Update: 2020-03-14