[1]卢婷,郑颖纯,杨海英,等.西藏小型猪乳牙牙体的组织结构特性[J].南方医科大学学报,2019,(09):1113.[doi:10.12122/j.issn.1673-4254.2019.09.18]
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西藏小型猪乳牙牙体的组织结构特性()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年09期
页码:
1113
栏目:
出版日期:
2019-09-15

文章信息/Info

Title:
Structural characteristics of the deciduous teeth of Tibetan miniature pigs
作者:
卢婷郑颖纯杨海英吴补领熊君黄呈潘育桦黎美怡何菲熊符
关键词:
西藏小型猪实验动物乳牙牙胚
Keywords:
Tibet miniature pigs laboratory animal model deciduous teeth tooth germ
DOI:
10.12122/j.issn.1673-4254.2019.09.18
摘要:
目的探索西藏小型猪乳牙牙体及牙胚的组织病理结构特性,为牙齿相关疾病及牙再生研究奠定基础。方法通过X线拍 摄西藏小型猪乳牙牙牙合系统结构,扫描电镜观察其乳牙牙釉质及牙本质超微结构,采用Micro-CT对乳牙进行扫描,Mimics软 件对颌骨及牙齿进行三维重建,进一步应用Image J 软件计算灰度值及计算乳牙矿化密度值,通过HE染色了解西藏小型猪牙 胚及牙髓组织结构特点。结果西藏小型猪乳牙牙冠由牙釉质、牙本质及牙髓组织构成。乳牙牙釉质和牙本质超微结构与人类 乳牙较一致,牙釉质和牙本质矿化密度值分别为2.47±0.09 g/cm3和1.72±0.07 g/cm3,牙胚和牙髓组织病理结构与人类牙齿结构 也较一致,乳牙牙髓组织处于未分化状态。结论西藏小型猪的乳牙解剖、超微结构、组织病理结构均与人类相似,是研究人类 牙体组织疾病发病及牙再生机制理想的动物模型。
Abstract:
Objective To explore the histological structure of the deciduous teeth and the tooth germs of Tibetan miniature pigs for studies of dental tissue diseases and tooth regeneration. Methods The structure of the deciduous teeth of Tibetan miniature pigs was observed by X-ray. The ultrastructure of the enamel and dentin of deciduous teeth was characterized by scanning electron microscopy. The jaws and teeth were three-dimensionally reconstructed using Mimics software based on Micro-CT scanning of the deciduous teeth. Image J software was used to calculate the gray value and the mineralization density of the deciduous teeth. Hisotological structure of the tooth germ and the pulp tissue of Tibetan miniature pigs was observed using HE staining. Results The deciduous teeth of Tibetan miniature pigs were composed of enamel, dentin and medullary pulp tissue. The permanent tooth germ were formed during the deciduous dentition. The enamel and dentin ultrastructure of deciduous teeth were consistent with that of human deciduous teeth. The enamel and dentin mineralization densities were 2.47±0.09 g/cm3 and 1.72±0.07 g/cm3, respectively. The pathological structures of tooth germ and pulp tissue were similar to those of human teeth, and the pulp tissue of the deciduous teeth was in an undifferentiated state. Conclusion The deciduous teeth of Tibetan miniature pig have similar anatomy, ultrastructure and histopathological structure to human teeth and can serve as a good animal model for studying human dental tissue diseases and the mechanisms of tooth regeneration.

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更新日期/Last Update: 1900-01-01