[1]罗起胜,罗宏成,符黄德,等.姜黄素通过抑制HDGF/β-catenin复合物降低胶质瘤细胞的侵袭性[J].南方医科大学学报,2019,(08):911.[doi:10.12122/j.issn.1673-4254.2019.08.06]
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姜黄素通过抑制HDGF/β-catenin复合物降低胶质瘤细胞的侵袭性()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年08期
页码:
911
栏目:
出版日期:
2019-08-31

文章信息/Info

Title:
Curcumin suppresses invasiveness and migration of human glioma cells in vitro by inhibiting HDGF/β-catenin complex
作者:
罗起胜罗宏成符黄德黄海能黄华东罗琨祥李传玉胡仁统郑传华蓝川琉唐乾利
关键词:
姜黄素胶质瘤细胞侵袭HDGFβ-catenin
Keywords:
curcumin glioma cell invasion hepatoma-derived growth factor β-catenin
DOI:
10.12122/j.issn.1673-4254.2019.08.06
摘要:
目的探讨姜黄素对胶质瘤细胞的侵袭、迁徙能力的影响及相关机制。方法四甲基偶氮唑蓝实验筛选合适的姜黄素药物 浓度;Transwell小室、Boyden小室及划痕实验检测姜黄素对胶质瘤细胞侵袭、迁移能力的影响;蛋白免疫印迹实验和蛋白质免 疫共沉淀实验检测相关蛋白质表达水平变化及相关蛋白质之间相互作用。结果终浓度为20 μmol/L的姜黄素培养基培养48 h 作为实验组处理因素;姜黄素能明显降低胶质瘤细胞的侵袭、迁移能力(P<0.05);机制分析显示,与空白对照组相比,姜黄素处 理组HDGF、N-cadherin、Vimentin、Snail、Slug蛋白表达水平下降,E-cadherin蛋白表达水平升高;在姜黄素处理过的胶质瘤细胞 中干扰和过表达HDGF可分别协同抑制和逆转上皮细胞-间充质转化(EMT)信号;姜黄素可以明显降低HDGF与β-catenin的结 合量。结论姜黄素通过抑制HDGF/β-catenin复合物,降低了EMT信号,从而抑制了胶质瘤细胞侵袭、迁移能力。
Abstract:
Objective To investigate the effect of curcumin on the invasion and migration of human glioma cells in vitro and explore the molecular mechanisms. Methods MTT assay was used for screening the optimal curcumin concentrations. The effects of curcumin on the invasion and metastasis of human glioma cell lines U251 and LN229 were tested using Transwell assay, Boyden assay and wound-healing assays. The expression of the related proteins and their interactions were determined using Western blotting and coimmunoprecipitation assay. Results Curcumin at the concentration of 20 μmol/L for 48 h was used as the optimal condition for subsequent cell treatment. In the two glioma cell lines, curcumin significantly suppressed the invasion and migration of the cells (P<0.05) and lowered the expressions of hepatoma-derived growth factor (HDGF), Ncadherin, vimentin, Snail and Slug, but increased the expression of E-cadherin. Interference of HDGF in curcumin-treated glioma cells synergistically inhibited the epithelial-mesenchymal transition (EMT) signals, while overexpression of HDGF significantly reversed the inhibitory effect of curcumin on EMT; curcumin treatment could significantly reduce the binding of HDGF to β-catenin. Conclusion Curcumin suppresses EMT signal by reducing HDGF/β-catenin complex and thereby lowers the migration and invasion abilities of human glioma cells in vitro.

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更新日期/Last Update: 1900-01-01