[1]杨海帆,谢建刚,张金铭,等.抑制miR-186表达可减轻缺氧状态下血管内皮细胞线粒体的损伤[J].南方医科大学学报,2019,(08):898.[doi:10.12122/j.issn.1673-4254.2019.08.04]
点击复制

抑制miR-186表达可减轻缺氧状态下血管内皮细胞线粒体的损伤()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年08期
页码:
898
栏目:
出版日期:
2019-08-31

文章信息/Info

Title:
Inhibiting miR-186 expression alleviates mitochondrial damage in hypoxic human umbilical vein endothelial cells
作者:
杨海帆谢建刚张金铭常远韩静
关键词:
miR-186缺氧诱导因子-1α血管内皮细胞线粒体损伤
Keywords:
miR-186 hypoxia-inducible factor-1α vascular endothelial cells mitochondrial damage
DOI:
10.12122/j.issn.1673-4254.2019.08.04
摘要:
目的探讨抑制miR-186表达对血管内皮细胞缺氧诱导因子-1α表达的影响和缺氧状态下线粒体功能的影响。方法构建 人脐静脉内皮细胞缺氧培养模型,检测正常培养组与缺氧培养6 h 组人脐静脉内皮细胞(HUVECs)miR-186 表达量。干扰组 HUVECs细胞转染miR-186-inhibit 48 h,干扰miR-186表达,对照组转染对照inhibit。检测miR-186的表达以确定干扰效果,干 扰成功后缺氧培养6 h后观测实验组和对照组电镜下线粒体结构。干扰miR-186表达后检测缺氧诱导因子-1α的mRNA和蛋白 表达。结果缺氧培养6 h 的HUVECs 细胞中miR-18 表达轻度上升(P=0.0188)。转染miR-186inhibit 48 h 可干扰miR-186 70%~80%的表达,干扰miR-186表达促进缺氧诱导因子-1α的mRNA和蛋白表达。干扰组缺氧培养6 h,电镜下线粒体损伤明显 减轻(P=0.00297)。结论干扰miR-186通过促进HIF-α上调,改善线粒体损伤,保护血管内皮细胞。靶向干扰miR-186可能有 助于失血性休克的治疗。
Abstract:
Objective To investigate the effect of miR-186 inhibition on the expression of hypoxia-inducible factor-1α (HIF-α) and mitochondrial function in hypoxic vascular endothelial cells. Methods Human umbilical vein endothelial cells (HUVECs) cultured in routine or hypoxic conditions for 6 h were examined for the expression of miR-186. A miR-186 inhibitor was transfected in the HUVECs, and the cells were subsequently cultured in hypoxic condition for 6 h to observe the changes in the mitochondrial structure under an electron microscope. The changes in the mRNA and protein expressions of HIF-1α in response to miR-186 interference were tested using real-time fluorescent quantitative PCR and Western blotting. Results The expression of miR-18 was mildly increased in HUVECs after hypoxic exposure for 6 h (P=0.0188). Interference of miR-186 expression obviously promoted the mRNA and protein expressions of HIF-1α in HUVECs. In hypoxic conditions, miR-186 interference significantly reduced mitochondrial damage in HUVECs as observed under electron microscope (P=0.0297). Conclusion Inhibition of miR-186 protects vascular endothelial cells against hypoxic injuries by promoting HIF-α expression to lessen mitochondrial damage, suggesting the possibility of targeted miR-186 interference for treatment of hemorrhagic shock.

相似文献/References:

[1]邵志强,郑少斌,肖耀军,等.缺氧诱导因子-1α和血管内皮生长因子在肾细胞癌组织中的表达及意义[J].南方医科大学学报,2005,(08):1034.
 SHAO Zhi-qiang,ZHENG Shao-bin,XIAO Yao-jun,et al.Expressions of hypoxia inducible factor-1α and vascular endothelial growth factor in human renal cell carcinoma[J].Journal of Southern Medical University,2005,(08):1034.
[2]陈卫东,常保超,张燕,等.雷公藤多苷对糖尿病大鼠肾组织缺氧诱导因子-1α及内皮素-1表达的影响[J].南方医科大学学报,2015,(04):499.

更新日期/Last Update: 1900-01-01