[1]郭远波,王研,张登文,等.乌司他丁对异氟烷介导的大鼠海马神经元凋亡的影响[J].南方医科大学学报,2019,(07):850.[doi:10.12122/j.issn.1673-4254.2019.07.16]
点击复制

乌司他丁对异氟烷介导的大鼠海马神经元凋亡的影响()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年07期
页码:
850
栏目:
出版日期:
2019-07-15

文章信息/Info

Title:
Effect of ulinastatin on isoflurane-induced neuronal apoptosis in the hippocampus of rats
作者:
郭远波王研张登文崔灿李涛王晟
关键词:
乌司他丁异氟烷海马细胞凋亡线粒体
Keywords:
ulinastatin isoflurane hippocampus apoptosis mitochondria
DOI:
10.12122/j.issn.1673-4254.2019.07.16
摘要:
目的探讨乌司他丁预处理对异氟烷介导的大鼠海马神经元线粒体途径凋亡的影响及可能的机制。方法36只SD雄性 大鼠随机分为对照组、异氟烷组和乌司他丁组,每组12只。对照组不给与任何处理,异氟烷组和乌司他丁组采用0.75%异氟烷 急性暴露6 h,而乌司他丁组在采用0.75%异氟烷急性暴露前,先给与50000 U/kg乌司他丁预处理。以TUNEL染色检测细胞凋 亡,JC-1探针检测线粒体膜电位(△ψm),Western blot检测细胞色素C释放及caspase-3活性,H2DCFDA探针检测细胞内活性氧 (ROS)。结果与对照组比较,异氟烷组海马神经元凋亡显著增加(P<0.05),而乌司他丁组显著下降(P<0.05);异氟烷组神经元 线粒体△ψm显著降低(P<0.05),乌司他丁组显著提高(P<0.05);异氟烷组海马神经元ROS、细胞色素C释放及caspase-3活性均 显著增加(P<0.05),而乌司他丁组显著降低(P<0.05)。结论乌司他丁可以抑制异氟烷介导的大鼠海马神经元凋亡,其机制可 能与抑制线粒体途径凋亡有关。
Abstract:
Objective To investigate the effect of ulinastatin pretreatment on isoflurane-induced mitochondria-dependent neuronal apoptosis in the hippocampus of rats. Methods Thirty-six male SD rats were randomly assigned into control group, isoflurane group and ulinastatin group. In the latter two groups, the rats were subjected to acute exposure to 0.75% isoflurane for 6 h and pretreated with 50 000 U/kg of ulinastatin before isoflurane exposure, respectively. After the treatments, apoptosis of the hippocampal neurons was detected using TUNEL assay, and the mitochondrial membrane potential ( △ ψm) was measured using JC-1 mitochondrial membrane potential kit; cytochrome C release and caspase-3 activity were examined with Western blotting, and intracellular reactive oxygen species (ROS) was detected using the fluorescent probe H2DCFDA. Results Compared with those in the control group, the rats with acute exposure to isoflurane showed markedly increased TUNELpositive cells in the hippocampus (P<0.05), which were obviously reduced by ulinastatin pretreatment (P<0.05). The △ψm of the hippocampal neurons was significantly reduced after isoflurane exposure (P<0.05), and was partly recovered by ulinastatin pretreatment (P<0.05). Acute exposure to isoflurane resulted in obviously increased cellular ROS, cytochrome C release and caspase-3 activity in the hippocampal neurons (P<0.05), and these changes were significantly inhibited by ulinastatin pretreatment (P<0.05). Conclusion Ulinastatin pretreatment provides neuroprotection against isoflurane-induced apoptosis of the hippocampal neurons in rats possibly by inhibiting mitochondria-dependent apoptosis pathway.

相似文献/References:

[1]郭培培,陈仲清,席小丽,等.肠道蛋白酶在失血性休克大鼠炎症反应中的作用[J].南方医科大学学报,2011,(06):1086.
[2]郭剑颖,邓群,郭旭升,等.乌司他丁和胸腺肽α1联合治疗严重脓毒症的组织病理学观察[J].南方医科大学学报,2012,(06):830.
[3]朱宇麟,周荣胜,杨会,等.乌司他丁对肝大部切除合并缺血再灌注损伤后肝再生及TNF-α/IL-6/STAT-3 信号通路的影响[J].南方医科大学学报,2012,(09):1301.
[4]李斌飞,廖小卒,程周,等.乌司他丁在体外膜肺氧合中的肝保护作用[J].南方医科大学学报,2012,(10):1511.
[5]彭娜,向定成,罗国新,等.乌司他丁对脓毒症休克大鼠心功能及p38MAPK活化的影响[J].南方医科大学学报,2012,(11):1620.
[6]陈 力,徐世元,李传翔,等.乌司他丁对老年人胃肠道大手术围术期心脏功能的影响[J].南方医科大学学报,2014,(01):117.
[7]郭晓华,王郑莲,刘亚楠,等.血液滤过联合乌司他丁治疗脓毒性休克的分子机制[J].南方医科大学学报,2015,(08):1189.
[8]周耿标,徐秋林,刘亚楠,等.乌司他丁对重症中暑小鼠急性肺损伤的保护作用[J].南方医科大学学报,2015,(09):1277.
[9]王显悦,董文鹏,童光,等.大剂量乌司他丁对A型主动脉夹层全弓置换患者的脏器保护作用[J].南方医科大学学报,2016,(08):1085.
[10]唐锟,刘畅,陈林,等.乌司他丁通过上调PPAR-γ抑制低氧诱导的肺血管平滑肌细胞表型转换[J].南方医科大学学报,2016,(10):1322.

更新日期/Last Update: 1900-01-01