[1]罗东,汪威,陈俊林,等.低强度脉冲超声对阿霉素联合环磷酰胺化疗后大鼠造血功能的影响[J].南方医科大学学报,2019,(07):836.[doi:10.12122/j.issn.1673-4254.2019.07.14]
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低强度脉冲超声对阿霉素联合环磷酰胺化疗后大鼠造血功能的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年07期
页码:
836
栏目:
出版日期:
2019-07-15

文章信息/Info

Title:
Effects of low-intensity pulsed ultrasound on hematopoietic function in rats after combined chemotherapy with doxorubicin and cyclophosphamide
作者:
罗东汪威陈俊林刘宝茹陈锦云王嫣陈文直
关键词:
低强度脉冲超声阿霉素环磷酰胺化疗
Keywords:
low-intensity pulsed ultrasound adriamycin cyclophosphamide chemotherapy
DOI:
10.12122/j.issn.1673-4254.2019.07.14
摘要:
目的探讨低强度脉冲超声(LIPUS)对阿霉素联合环磷酰胺化疗后大鼠造血功能的影响。方法将80只大鼠根据性别和 体质量进行随机分层分组,对照组和LIPUS组各40只。对照组用阿霉素(2 mg/kg)+环磷酰胺(20 mg/kg)先后腹腔注射,连续给 药4 d;LIPUS组在相同剂量给药后LIPUS连续辐照7 d。于开始给药第0、4、7、9、11、14、18天测定各组大鼠白细胞、红细胞、血 小板计数;于第0、4、11天做骨髓组织病理切片、流式细胞术检测凋亡、qPCR(SCF、ICAM-1、VCAM-1)检测、ELISA检测IL-3和 GM-CSF。结果血常规结果显示,LIPUS组白细胞较对照组明显提高(P<0.05),骨髓组织病理切片结果显示LIPUS组造血组 织的增加显著(P<0.05),流式细胞术结果显示LIPUS组骨髓细胞凋亡低于对照组(P<0.05),qPCR结果显示LIPUS组ICAM-1 和VCAM-1的水平明显高于对照组(P<0.05),ELISA检测结果显示,LIPUS组IL-3和GM-CSF均显著高于对照组(P<0.05)。结 论LIPUS可改善阿霉素联合环磷酰胺化疗后的造血损伤,可能是因为其能提升ICAM-1、VCAM-1水平和IL-3、GM-CSF水平 所致。
Abstract:
Objective To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on hematopoietic function in rats after combined chemotherapy with doxorubicin and cyclophosphamide. Methods Eighty rats were randomized into control group and LIPUS group (n=40) for treatment with intraperitoneal injection of doxorubicin (2 mg/kg)+cyclophosphamide (20 mg/kg) for 4 consecutive days and continuous irradiation with LIPUS for 7 days following the injections, respectively. The white blood cells, red blood cells and platelets counts in each group were measured at 0, 4, 7, 9, 11, 14 and 18 days after the start of drug administration. The pathological sections of the bone marrow were examined at 0, 4 and 11 days, and the flow cytometry was performed for detecting the cell apoptosis; qPCR was performed for detecting the expressions of SCF, ICAM-1, and VCAM-1 mRNAs, and ELISA was used to detect the expressions of IL-3 and GM-CSF. Results The white blood cell count was significantly higher in LIPUS group than in the control group (P<0.05). Histopathological examination of the bone marrow revealed significantly increased hematopoietic tissue in LIPUS group (P<0.05). Flow cytometry demonstrated an obviously lower cell apoptosis rate in the bone marrow in LIPUS group than in the control group (P<0.05). Compared with those in the control group, the mRNA expression levels of ICAM-1 and VCAM-1 as well as the protein levels of IL-3 and GM-CSF were significantly increased in LIPUS group (P<0.05). Conclusion LIPUS can alleviate the hematopoietic damage after combined chemotherapy with doxorubicin with cyclophosphamide probably by increasing the expressions of ICAM-1, VCAM-1, IL-3, and GM-CSF.

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更新日期/Last Update: 1900-01-01