[1]黄云,刘永泓,辛学刚.高频超声和光学相干断层成像可在体监测裸鼠黑素瘤的动态发展过程[J].南方医科大学学报,2019,(07):772.[doi:10.12122/j.issn.1673-4254.2019.07.04]
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高频超声和光学相干断层成像可在体监测裸鼠黑素瘤的动态发展过程()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年07期
页码:
772
栏目:
出版日期:
2019-07-15

文章信息/Info

Title:
Dynamic imaging of melanoma development in nude mice using high-frequency ultrasound and optical coherence tomography
作者:
黄云刘永泓辛学刚
关键词:
皮肤黑素瘤生长过程光学相干断层成像高频超声
Keywords:
cutaneous melanoma growth process optical coherence tomography high-frequency ultrasound
DOI:
10.12122/j.issn.1673-4254.2019.07.04
摘要:
目的探讨高频超声和光学相干断层成像(OCT)在监测皮肤黑素瘤生长过程中的成像效果。方法将20只4周龄的裸鼠 随机分为实验组(n=16)与对照组(n=4)。实验组:将0.2 mLA375人源皮肤黑素瘤细胞悬液经皮下种植于裸鼠背部;对照组:将 等量的培养液经皮下种植于裸鼠背部。自接种后起,每天对裸鼠进行大体观察、小动物高频超声成像以及OCT成像,对所得图 像进行量化分析,并记录肿瘤成瘤时间、肿瘤大小、体积、长径、厚径等信息,接种后第24天解剖裸鼠,切取肿瘤块进行病理检 查。结果皮肤黑素瘤接种成功率为87.5%,结果经HE染色证实。OCT比高频超声更早探测到肿瘤。随着肿瘤的生长,在高频 超声图像中可见点状或带状强回声信号,在OCT图像中出现真皮上层的扁平化,并且在高频超声与OCT中,CNR均逐步增强 (P<0.05)。生长曲线图显示,与高频超声相比,OCT对厚径的测量更灵敏,测量结果比较差异有统计学意义(P<0.05)。结论高 频超声和OCT可在体监测皮肤黑素瘤的动态发展过程,且在不同发展阶段,成像效果不同。高频超声和OCT在肿瘤生长的不 同阶段的影像学表现,可在研究肿瘤动态发展过程中,对不同影像模态的选择提供参考依据。
Abstract:
Objective To investigate the application of high- frequency ultrasound (HFUS) and optical coherence tomography (OCT) for monitoring the progression of cutaneous melanoma (CM) in nude mice. Methods Twenty 4-week-old nude mice were randomly divided into CM group (n=16) and control group (n=4). In CM group, A375 cell suspension were injected subcutaneously on the back of the nude mice, and only culture medium was injected in the control group. The tumor growth was monitored by gross observation and with HFUS and OCT on a daily basis. The tumor formation rate, time, and size were recorded and melanoma visibility was assessed quantitatively using the contrast- to-background-noise ratio (CNR). Twentyfour days after cell implantation, the tumors were dissected for pathological examination. Results The tumor formation rate was 87.5% in CM group. OCT detected tumor formation at an earlier time than HFUS. With the growth of the tumor, HFUS detected spots or bands of strong echoes, and flattening of the upper dermis could be observed in OCT; the of HFUS and OCT were obviously enhanced over time (P<0.05). The tumor growth curve showed that OCT was more sensitive for measurement of the tumor thickness than HFUS. The length, depth and volume of the tumors measured by HFUS were significantly greater than those measured by OCT (P<0.05). Conclusion HFUS and OCT can monitor the dynamic development of cutaneous melanoma in nude mice, and their imaging performance differs in different stages of tumor development.
更新日期/Last Update: 1900-01-01