[1]周芝熠,李晓娟,蒋国庆,等.维生素D通过下调miR-21的表达促进人胎盘滋养细胞的迁移和侵袭[J].南方医科大学学报,2019,(04):437.[doi:10.12122/j.issn.1673-4254.2019.04.09]
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维生素D通过下调miR-21的表达促进人胎盘滋养细胞的迁移和侵袭()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年04期
页码:
437
栏目:
出版日期:
2019-04-15

文章信息/Info

Title:
Vitamin D down-regulates microRNA-21 expression to promote human placental trophoblast cell migration and invasion in vitro
作者:
周芝熠李晓娟蒋国庆王珏钱源
关键词:
维生素DmiR-21HTR-8/SVneo迁移人胎盘滋养细胞
Keywords:
vitaminD microRNA-21 trophoblasts HTR-8/SVneo cells migration human placental trophoblast cells
DOI:
10.12122/j.issn.1673-4254.2019.04.09
摘要:
目的探讨维生素D对microRNA-21表达的调控作用以及对人胎盘滋养细胞迁移、侵袭能力的影响。方法不同剂量维生 素D刺激HTR-8/SVneo细胞24、48、72 h后,RT-qPCR 检测microRNA-21表达情况;将miR-21 mimic、miR-21 mimic-nc、miR- 21 inhibitor和miR-21 inhibitor-nc分别转染至HTR-8/SVneo细胞,或将miR-21 mimic、miR-21 inhibitor联合维生素D共同刺激 细胞,Transwell实验检测细胞的迁移和侵袭能力,western blot 检测E-cadherin、Fibronectin、MMP9的蛋白表达水平。结果维 生素D刺激HTR-8/SVneo细胞后抑制了micoRNA-21的表达,且抑制作用呈浓度-时间依赖性增强。单独转染miR-21 mimic抑 制HTR-8/SVneo细胞的迁移、侵袭能力,E-cadherin表达水平增加,Fibronectin、MMP9表达水平降低;联合维生素D共同刺激细 胞则可以减弱该作用。单独转染miR-21 inhibitor 促进了HTR-8/SVneo 细胞的迁移、侵袭能力,E-cadherin 表达水平降低, Fibronectin、MMP9表达水平增加;联合维生素D共同作用与单细胞独转染miR-21 inhibitor相比,细胞迁移、侵袭能力以及蛋白 水平无明显改变。结论维生素D可能通过下调microRNA-21的表达来影响子痫前期发生发展。
Abstract:
Objective To investigate the effect of vitamin D on microRNA-21 (miR-21) expression and migration and invasion of human placental trophoblast cells. Methods The changes in the expression of miR-21 were detected using RT-qPCR in HTR-8/ SVneo cells following stimulation by vitamin D at different doses for 24, 48 and 72 h. HTR-8/SVneo cells transfected with miR-21 mimic or inhibitor with or without vitamin D treatment were examined for changes in cell migration and invasion abilities using Transwell assay, and Western blotting was used to detect protein expressions of E-cadherin, fibronectin, and MMP9. Results Vitamin D obviously inhibited the expression of micoRNA-21 in HTR-8/SVneo cells in a concentration-and time- dependent manner. Transfection with the miR-21 mimic significantly inhibited the migration and invasion of HTR-8/ SVneo cells, and this inhibitory effect was abolished by treatment with vitamin D; transfection with miR-21 inhibitor obviously promoted the migration and invasion of HTR-8/SVneo cells, and these effects were not significantly affected by vitamin D treatment. Conclusion Vitamin D may promote trophoblast cell migration and invasion to accelerate the development of preeclampsia by down-regulating the expression of miR-21.

相似文献/References:

[1]李晓青,陈鑫,黄珊,等.下调miR-21可以增强食管癌TE-1细胞的放射敏感性[J].南方医科大学学报,2012,(11):1559.
[2]许瑞,黄华萍,韩忠,等.miR-21 靶向TLR-4/MyD88信号通路介导冷空气诱导的气道免疫失调[J].南方医科大学学报,2016,(01):98.

更新日期/Last Update: 1900-01-01