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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:

期数:

2018年11期

页码:

1384

栏目:

出版日期:

2018-11-30

文章信息/Info

Title:

Effect of electro-acupuncture at Zusanli acupoint on postoperative T cell immune function in rats

作者:

张建杏; 王研; 郭远波; 纪雪霞; 王晟

关键词:

电针; 足三里; 手术; 细胞免疫

Keywords:

electroacupuncture;  Zusanli;  operation;  T cell immunity

DOI:

10.12122/j.issn.1673-4254.2018.11.18

摘要:

目的研究电针足三里穴调节大鼠围术期细胞免疫功能的作用。方法选取42只SD大鼠，分为3组，空白组（6只）、模型组 （18只）、电针组（18只），模型组及电针组麻醉后行大腿切开并股骨离断术，电针组于麻醉前及手术后1 h，在双侧足三里穴予以 电针刺激15 min，空白组不予任何处理。模型组及电针组分别于术后6、24、72 h称重处死，取SD大鼠心室血，流式细胞仪检测 CD3、CD4、CD8的含量并计算CD4/CD8，ELISA检测IL-1、IL-6，并与空白组比较。结果模型组及电针组CD3在术后6、24 h较 空白组均明显下降，模型组在术后72 h较空白组仍低，电针组在术后72 h已恢复至空白组水平，在各相应时间点，模型组CD3水 平均明显低于电针组；模型组CD4在术后6、24 h较空白组明显下降，至术后72 h才基本恢复至空白组水平，电针组CD4在术后 6 h较空白组明显下降，但术后24 h已恢复至空白组水平，在术后24、72 h，模型组CD4水平均低于电针组；模型组及电针组CD8 在术后6、24、72 h 较空白组均无明显变化；模型组CD4/CD8 术后24、72 h 较空白组明显下降，电针组则无明显变化，术后24、 72 h，模型组及电针组CD4/CD8有明显差异，模型组低于电针组。模型组及电针组IL-1水平在术后6、24、72 h较空白组均明显 下降，在各相应时间点，模型组IL-1水平均明显低于电针组；模型组及电针组IL-6水平在术后6、24 h较空白组均明显升高，术后 72 h电针组已降低，模型组仍升高，在术后6、72 h模型组IL-6水平均明显高于电针组。结论电针组细胞免疫功能的抑制程度 明显较模型组轻，且恢复明显比模型组快，表明电针足三里穴对大鼠术后细胞免疫功能具有一定的保护作用，有利于术后恢复。

Abstract:

Objective To study the effect of electro- acupuncture at Zusanli acupoint in regulating perioperative cell immune functions in rats. Methods Forty-two SD rats were divided into blank control group (n=6), model group (n=18), and electroacupuncture group (n=18). The rats in the latter two groups underwent thigh incision and femoral dissection under anesthesia; the rats in electro-acupuncture group received electro-acupuncture at bilateral Zusanli acupoint for 15 min before anesthesia and 1 h after the surgery. The rats in the model group and electro-acupuncture group were sacrificed at 6 h, 24 h, and 72 h after the operation and blood samples were taken from the ventricle for analyzing CD3, CD4, and CD8 T cell subpopulations and calculation of CD4/CD8 using flow cytometry. ELISA was used to detect the levels of interleukin-1 (IL-1) and IL-6. Results The CD3 T cell subpopulation was significantly lower in the model group and electro-acupuncture group than in the blank group at 6 h and 24 h after the operation. At 72 h after the operation, CD3 subpopulation levels still remained low in the model group, but recovered the control level in electro-acupuncture group. At each time point of measurement, CD3 level was significantly lower in the model group than in the electro-acupuncture group. CD4 level in the model group was significantly lowered at 6 h and 24 h after the operation, and recovered the control level at 72 h. In the electro-acupuncture group, CD4 level was significantly lowered at 6 h after the operation, but recovered the control level at 24 h. At 24 h and 72 h, the levels of CD4 were significantly lower in the model group than in the electro-acupuncture group. CD8 level underwent no significant changes after the operation in either the model group or electro-acupuncture group. CD4/CD8 was significantly lowered at 24 h and 72 h after the operation in the model group but showed no significant variation in the electro-acupuncture group. Compared with that in the control group, IL-1 level was significantly lowered in both the model group and electroacupuncture group at 6 h, 24 h, and 72 h after the operation, and was significantly lower in the model group than in the electroacupuncture group at these time points. IL-6 level increased significantly in the model group and the electro- acupuncture group at 6 h and 24 h. at 72 h, IL-6 level was obviously lowered in the electro-acupuncture group but remained elevated in the model group. Conclusion Electro-acupuncture alleviates postoperative immune suppression and promotes recovery of the immune function in rats, suggesting a protective effect of electro-acupuncture at Zusanli acupoint on cellular immune function after surgery.

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