[1]王允,张玉媛,陈雪,等.联合应用肌醇和木樨草素可选择性抑制肺腺癌A549细胞的生长[J].南方医科大学学报,2018,(11):1378.[doi:10.12122/j.issn.1673-4254.2018.11.17]
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联合应用肌醇和木樨草素可选择性抑制肺腺癌A549细胞的生长()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年11期
页码:
1378
栏目:
出版日期:
2018-11-30

文章信息/Info

Title:
Combined treatment with myo-inositol and luteolin selectively suppresses growth of human lung cancer A549 cells possibly by suppressing activation of PDK1 and Akt
作者:
王允张玉媛陈雪洪运吴征东
关键词:
肌醇木樨草素A549细胞联合应用化学预防
Keywords:
myo-inositol luteolin A549 cells combined treatment chemoprevention
DOI:
10.12122/j.issn.1673-4254.2018.11.17
摘要:
目的研究肌醇(MI)和木樨草素(LUT)对人肺腺癌A549细胞的作用,并探讨其机制。方法不同浓度的肌醇和木樨草素 处理A549细胞24 h或48 h,MTT法检测细胞活性。10 mmol/L 肌醇和20 μmol/L 木樨草素单独或共同处理A549细胞和永生化 肺支气管上皮细胞Beas-2B 48 h,MTT法、平板克隆形成实验和划痕愈合实验分别检测肌醇和木樨草素对两种细胞活性、细胞 克隆形成和迁移的影响,Western blot法检测肌醇和木樨草素对A549细胞中相关蛋白质表达水平的影响。结果肌醇和木樨草 素均可抑制A549细胞的活性,且呈浓度依赖性。10 mmol/L 肌醇和20 μmol/L 木樨草素作用A549细胞48 h后,肌醇处理组、木 樨草素处理组和联合处理组A549细胞的活性分别是对照组的92%、83%和70%,平板克隆形成数分别是对照组的79%、73%和 43%,划痕愈合度分别为24.61%、13.08%和8.65%,对照组划痕愈合度为29.99%;同等剂量下的肌醇和木樨草素单独或共同处 理对Beas-2B细胞的活性、克隆形成和迁移没有明显的抑制作用。与对照组相比,肌醇处理组细胞中p-PDK1和p-Akt以及木樨 草素处理组细胞中p-PDK1的蛋白质表达明显减少(P<0.05),联合处理组下调的幅度更为明显(P<0.05)。结论肌醇和木樨草 素可选择性的抑制A549细胞的活性、克隆形成和迁移,合用时抑制作用更为明显,可能是通过下调p-PDK1和p-Akt的表达发 挥的作用。
Abstract:
Objective To study the effects of myo-inositol and luteolin on human lung cancer A549 cells and explore the possible mechanisms. Methods A549 cells were treated with different concentrations of myo- inositol and luteolin, either alone or in combination, and the cell viability was examined using MTT assay. A549 cells and human bronchial epithelial Beas-2B cells were treated for 48 h with 10 mmol/L myo-inositol and 20 μmol/L luteolin, alone or in combination, and the cell proliferation was detected using MTT assay; the colony formation and migration of the cells were examined with colony formation assay and wound healing assay, respectively. The protein expression levels in A549 cells were detected using Western blotting. Results Both myo-inositol and luteolin could dose-dependently inhibit the viability of A549 cells. Treatments with 10 mmol/L myo-inositol, 20 μmol/L luteolin, and both for 48 h caused significant reduction in the cell viability (92%, 83% and 70% of the control level, respectively) and colony number (79%, 73% and 43%, respectively), and significantly lowered the wound closure rate (24.61%, 13.08% and 8.65%, respectively, as compared with 29.99% in the control group). Similar treatments with myoinositol and luteolin alone or in combination produced no significant inhibitory effect on the growth, colony formation or migration of Beas-2B cells. The expressions of p-PDK1 and p-Akt in myo-inositol-treated A549 cells and the expression of p- PDK1 in luteolin-treated cells were significantly decreased (P<0.05), and the decrements were more obvious in the combined treatment group (P<0.05). Conclusion Luteolin combined with myo-inositol can selectively inhibit the proliferation and migration of A549 cells, and these effects are probably mediated, at least in part, by suppressing the activation of PDK1 and Akt.

相似文献/References:

[1]黄芳,曲彩红,吴惠勤,等.气相色谱-质谱法测定螺旋藻中肌醇的含量[J].南方医科大学学报,2006,(07):1075.

更新日期/Last Update: 1900-01-01