[1]刘慧慧,刘嘉羿,彭美玉,等.针刺对气道重塑小鼠TGF-β1/Smads通路的影响[J].南方医科大学学报,2018,(11):1372.[doi:10.12122/j.issn.1673-4254.2018.11.16]
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针刺对气道重塑小鼠TGF-β1/Smads通路的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年11期
页码:
1372
栏目:
出版日期:
2018-11-30

文章信息/Info

Title:
Effect of acupuncture on TGF-β1/Smads pathway in mice with airway remodeling mic
作者:
刘慧慧刘嘉羿彭美玉黎玉辉李春桥
关键词:
针刺气道重塑TGF-β1/Smads哮喘
Keywords:
acupuncture airway remodeling transforming growth factor-β1/Smads asthma
DOI:
10.12122/j.issn.1673-4254.2018.11.16
摘要:
目的探讨针刺对气道重塑小鼠肺组织TGF-β1/Smads信号通路的调控作用。方法将30只SPF 级小鼠随机分为空白组、 模型组、针刺组,10只/组。模型组、针刺组制作小鼠哮喘模型。针刺组自造模之日起第10天行针刺操作,穴取肺俞、大椎、足三 里(两侧),每次留针20 min,隔日针刺1 次,共治疗7次。并于最后1次针刺结束后24 h行1%卵蛋白雾化吸入3 d,最后1次激发 后24 h于不同浓度乙酰甲基氯化胆碱(Mch)吸入下通过无创体积描计器检测小鼠肺阻力,然后采用HE染色法观察小鼠肺组织 的病理变化,ELISA 检测小鼠支气管肺泡灌洗液(BALF)及血清中TGF-β1的表达,Western blot对各组气道平滑肌中差异蛋白 进行检测。将针刺组分离提取气道平滑肌细胞,空白组加入PBS液,抑制组加入TGF-β1抑制剂(LY2157299)分别进行培养,然 后Western blot检测两组细胞type-I及Smads蛋白表达相对量。结果模型组小鼠气管痉挛明显,管腔狭窄,支气管黏膜增厚,部 分上皮细胞脱落,肺泡隔增厚,气道平滑肌明显增厚;针刺组较模型组有明显改善;不同浓度Mch 激发后模型组小鼠肺阻力最 高,较其它两组均差异有统计学意义(P<0.05)。且3组小鼠的肺阻力都随着Mch的浓度加大而增加。模型组血清中TGF-β1阳 性表达OD值较空白组增加(P<0.05);针刺组阳性表达低于模型组(P<0.05)。针刺组BALF 中TGF-β1 含量低于模型组(P< 0.05)高于空白组(P<0.05)。模型组气道平滑肌中α-SMA、TGF-β1 及Smads 蛋白表达相对量均高于针刺组及空白组(均P< 0.05),抑制组细胞中type-I及Smads蛋白表达相对量较空白组高(P>0.05)。结论针刺能通过抑制气道TGF-β1 的表达,下调 Smads及α-SMA的表达来抑制气道重塑,减轻哮喘气道炎性反应,且抑制TGF-β1后,type-I及Smads蛋白表达相对量较高,针刺 可以通过TGF-β1/Smads通路来控制哮喘进展,为临床上哮喘的治疗提供理论依据。
Abstract:
Objective To investigate the effect of acupuncture on TGF-β1/Smads signaling pathway in the lung tissue of mice with airway remodeling. Methods Thirty specific pathogen-free mice were randomly divided into blank group, model group and acupuncture group (n=10). Mouse models of asthma were established in the model group and the acupuncture group, and the mice in the latter group received 7 acupuncture therapies (at bilateral Fei Shu, Da Zhui and Zu Sanli, 20 min each time) every other day, starting on the 10th day after the modeling. At 24 h after the last acupuncture, the mice were subjected to inhalation of 1% OVA for 3 days, and 24 h after the last challenge, the mice were given methacholine chloride (Mch) inhalation at different concentrations for measurement of lung resistance using a noninvasive stroke volume meter. HE staining was used to observe the pathological changes in the lung tissues, and TGF-β1 levels in the the bronchoalveolar lavage fluid (BALF) and serum were detected using ELISA; Western blotting was used to detect the differential protein expressions in the airway smooth muscles between the two groups. The airway smooth muscle cells were isolated from the mice in the acupuncture group and treated with a TGF- β1 inhibitor (LY2157299), and the relative expressions of type- I and Smads proteins were detected using Western blotting. Results The mice in the model showed obvious tracheal fistula with airway pathologies including lumen narrowing, bronchial mucosa thickening, dissociation of the epithelial cells, and thickening of the alveolar septum and airway smooth muscles. These pathological changes were obviously milder in the acupuncture group. The asthmatic mice exhibited significantly increased lung resistance in positive correlation with Mch concentration. Serum TGF-β1 level was significantly elevated in asthmatic mice (P<0.05); TGF-β1 levels in the serum and BALF were significantly lower in the acupuncture group than in the model group (P<0.05). In the model group, the expressions of α-SMA, TGF-β1 and Smads in the airway smooth muscles were significantly higher than those in the other two groups (both P<0.05). In cultured airway smooth muscle cells, the expressions of type-I and Smads were significantly higher in cells treated with LY2157299 than in the control cells (P>0.05). Conclusion Acupuncture can inhibit airway remodeling by inhibiting the expression of airway TGF-β1 and down-regulating the expression of Smads and α-SMA to reduce airway inflammatory response. Airway expressions of type-I and Smads proteins remain high after inhibiting TGF-β1. Acupuncture may control asthma progression through the TGF-β1/Smads pathway.

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更新日期/Last Update: 1900-01-01