[1]郑吉龙,霍德民,王玖琳,等.兔死后角膜瞳孔区域图像颜色的时序性变化规律[J].南方医科大学学报,2018,(10):1266.[doi:10.12122/j.issn.1673-4254.2018.10.19]
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兔死后角膜瞳孔区域图像颜色的时序性变化规律()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年10期
页码:
1266
栏目:
出版日期:
2018-10-16

文章信息/Info

Title:
Temporal pattern of postmortem color changes in the pupil region of the cornea in rabbits
作者:
郑吉龙霍德民王玖琳赵开放腾月马钰
关键词:
角膜混浊计算机图像处理技术颜色特征死亡时间时序性变化
Keywords:
corneal opacity computer image processing technology color features postmortem interval temporal pattern
DOI:
10.12122/j.issn.1673-4254.2018.10.19
摘要:
目的探讨个体死后角膜瞳孔区域图像颜色时序性变化规律,为死亡时间(PMI)推断提供一种无损、客观方法。方法构建 家兔空气栓塞和溺死死亡模型,在温度20 ℃、湿度30%的暗室内,于死后72 h内每隔2 h用数码相机获取兔角膜图像。通过计 算机图像处理技术(MATLAB)分割出角膜瞳孔区域图像,提取RGBHSV 6项图像颜色特征参数值并进行与PMI关系的回归分 析,同时比较不同死因对死后角膜瞳孔区域图像颜色变化的影响。结果不同死因死后72 h内,兔角膜瞳孔区域图像的R、G、B 值均随PMI呈上升趋势,与PMI拟合关系较好(P<0.01),H、S、V值与PMI关系均无统计学意义(P>0.05);经比较,两种死因死后 兔角膜瞳孔区域图像R、G、B值的变化趋势一致,与PMI的相关性相近,空气栓塞组兔角膜瞳孔区域图像的R、G、B实测值总体 大于溺死组。结论个体死后角膜瞳孔区域图像颜色变化随PMI呈一定规律性,死因会影响死后角膜瞳孔区域图像颜色特征参 数值大小。研究建立的相关回归方程为无损、客观推断PMI提供参考依据。
Abstract:
Objective To explore the temporal pattern of postmortem color changes in the pupil region of the cornea for noninvasive estimation of the postmortem interval (PMI). Methods Two rabbit models of air embolism and drowning were established in a dark room at a temperature of 20 ℃ with a relative humidity of 30%. The corneal images of the rabbits were acquired using a digital camera at two- hour intervals within 72 h after death. The pupil region on the corneal images was segmented using computer image processing technique (MATLAB), and the parameters of 6 image color features (RGBHSV) were extracted. Regression analysis was used to analyze the relationship between these parameters and the PMI, and the effects of different death causes on the changes of the corneal color features were also assessed. Results Within 72 h after death from different causes, the R, G and B values of the pupil region on the corneal images all tended to increase with the PMI, showing a good fitting with the PMI (P<0.01). No significant correlation was found between the values of H, S and V and the PMI (P>0.05). The R, G and B values in the pupil region on the corneal images showed consistent variation trends after death from the two causes, and their correlations with PMI were also similar. The measured values of R, G and B in air embolism group were greater than those in the drowning group. Conclusion The postmortem color changes of the pupil region on corneal images follow an identifiable temporal pattern and can vary across different causes of death. The regression equations established in this study provide references for non-invasive and objective estimation of the PMI.
更新日期/Last Update: 1900-01-01