[1]刘川,林春水,郭培培,等.胚胎期斑马鱼丙泊酚暴露可下调髓鞘碱性蛋白的表达[J].南方医科大学学报,2018,(09):1115.[doi:10.12122/j.issn.1673-4254.2018.09.15]
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胚胎期斑马鱼丙泊酚暴露可下调髓鞘碱性蛋白的表达()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年09期
页码:
1115
栏目:
出版日期:
2018-09-30

文章信息/Info

Title:
Exposure to propofol down-regulates myelin basic protein expression in zebrafish embryos: its neurotoxicity on oligodendrocytes and the molecular mechanisms
作者:
刘川林春水郭培培张昕朱晓勤
关键词:
丙泊酚鞘磷脂蛋白少突胶质细胞凋亡斑马鱼胚胎
Keywords:
propofol myelin basic protein oligodendrocyte apoptosis zebrafish embryos
DOI:
10.12122/j.issn.1673-4254.2018.09.15
摘要:
目的探讨胚胎期斑马鱼丙泊酚暴露下调髓鞘碱性蛋白(MBP)表达及相关机制。方法受精后6~48 h(6~48 hpf)的斑马 鱼胚胎,随机分为对照组、二甲基亚砜(DMSO)组、20 μg/mL丙泊酚组和30 μg/mL丙泊酚组,采用浸泡方式给药。于48 hpf、 72 hpf 收集幼鱼。通过荧光定量RT-qPCR法检测MBP mRNA和少突胶质细胞转录因子1(Olig1)、Olig2、性别决定区盒基因 (Sox10)mRNA表达(n=80条/组);Western blot法检测MBP蛋白表达(n=80条/组);TUNEL、免疫荧光法检测少突胶质细胞凋亡 (n=6条/组)。结果与对照组相比,20 μg/mL、30 μg/mL丙泊酚暴露两组MBP mRNA、MBP蛋白于72 hpf均明显下调(P<0.05), 另外30 μg/mL 组较20 μg /mL 组MBP蛋白表达显著减少(P<0.05);20 μg /mL、30 μg /mL 两组转录因子Olig1、Olig2、Sox10 mRNA表达于48 hpf、72 hpf明显下调(P<0.05),少突胶质细胞凋亡于72 hpf显著增多(P<0.05)。结论胚胎期斑马鱼丙泊酚暴 露可能通过诱导少突胶质细胞凋亡增多及抑制转录因子Olig1、Olig2、Sox10,从而下调MBP表达。
Abstract:
Objective To investigate the mechanism underlying propofol- induced down-regulation of myelin basic protein (MBP) in zebrafish embryos. Methods Zebrafish embryos (6-48 h post-fertilization [hpf]) were randomized into 4 equal groups for exposure to dimethyl sulfoxide (DMSO), 20 μg/mL propofol, 30 μg/mL propofol, or no particular treatment (control group). The larvae were collected at 48 or 72 hpf for detecting the mRNA levels of MBP, Olig1, Olig2, and Sox10 using qRT-PCR (n=80). The protein expression of MBP was quantitatively detected using Western blotting (n=80), and the apoptosis of the oligodendrocytes was investigated using TUNEL staining (n=6). Results Exposure to 20 and 30 μg/mL propofol caused significant reductions in the mRNA expressions of Olig1, Olig2, and Sox10 at 48 and 72 hpf (P<0.05) and also in MBP mRNA and protein levels at 72 hpf (P<0.05). Exposure to 30 μg/mL propofol induced more obvious reduction in MBP protein expression than 20 μg/mL propofol at 72 hpf (P<0.05), and the exposures resulted in a significant increase of oligodendrocyte apoptosis at 72 hpf (P<0.05). Conclusion Propofol exposure reduces MBP expression at both the mRNA and protein levels in zebrafish embryos by down-regulating the expressions of Olig1, Olig2 and Sox10 mRNA levels and increasing apoptosis of the oligodendrocytes.

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更新日期/Last Update: 1900-01-01