[1]黄晓其,吴晓丽,颜思珊,等.蜂胶对Triton-WR1339所致高脂血症小鼠的降脂作用及调控脂质代谢机制[J].南方医科大学学报,2018,(08):1020.
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蜂胶对Triton-WR1339所致高脂血症小鼠的降脂作用及调控脂质代谢机制()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年08期
页码:
1020
栏目:
出版日期:
2018-07-31

文章信息/Info

Title:
Lipid-lowering effect of propolis in mice with Triton-WR1339-induced hyperlipidemia and its mechanism for regulating lipid metabolism
作者:
黄晓其吴晓丽颜思珊兰天
关键词:
蜂胶降血脂Triton-WR1339
Keywords:
propolis hypolipidemia Triton-WR1339 lipid metabolism
摘要:
目的探究蜂胶对Triton-WR1339所致高脂血症的降脂作用及调控脂质代谢机制。方法将C57BL/6小鼠随机分为7组, 10 只/组,雌雄各半,分别为正常组、模型组、非诺贝特组(30 mg/kg)、蜂胶HB01 高剂量组(60 mg/kg)、蜂胶HB01 低剂量组 (30 mg/kg)、蜂胶HB02高剂量组(60 mg/kg)和蜂胶HB02低剂量组(30 mg/kg)。采用肌肉注射Triton WR-1339试剂造成高脂 血症模型,灌胃1周后眼眶取血,离心后测定血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL)、低密度脂蛋白 胆固醇(LDL)、丙二醛(MDA)、总超氧化物歧化酶(SOD)、谷丙转氨酶(GPT)、谷草转氨酶(GOT)等指标;取肝脏组织,置-80 ℃ 保存,Western blot法测定脂质转运蛋白的表达水平。结果与正常组相比,模型组血清中TC、TG、LDL、MDA、GPT、GOT的含 量升高,HDL含量降低、SOD活力下降(P<0.05)。与模型组比较,阳性药(非诺贝特:30 mg/kg)和两种蜂胶(高剂量组:60 mg/kg; 低剂量组:30 mg/kg)都能明显降低TC、TG、LDL、MDA、GPT、GOT含量而升高HDL含量,促进SOD活力,差异有统计学意义 (P<0.05),且蜂胶的效果略优于阳性药组。与正常组相比,Triton-WR1339 诱导的模型组肝脏中的ABCA1、ABCG8、低密度 脂蛋白和SR-B1 等脂质转运蛋白显著下降,差异有统计学意义(P<0.05),但阳性药(非诺贝特:30 mg/kg)和蜂胶(高剂量组: 60 mg/kg;低剂量组:30 mg/kg)干预后均能逆转这一下降趋势,蜂胶组的干预效果略优于阳性药组,差异有统计学意义(P<0.05)。 结论蜂胶具有显著的降血脂作用,其作用机制与改善肝脏脂质代谢紊乱、调控脂质代谢转运蛋白相关。
Abstract:
Objective To evaluate the therapeutic effect of propolis against Triton-WR1339-induced hyperlipidemia in mice and explore the underlying mechanism. Methods C57BL/6 mice were randomly divided into 7 groups (n=10), including the control group, hyperlipidemia model group, fenofibrate (30 mg/kg) treatment group, and 4 treatment groups treated with low- (30 mg/kg) or high-dose (60 mg/kg) propolis HB01 or HB02. In all but the control group, acute hyperlipidemia models were established by intramuscular injection of Triton WR-1339, and corresponding treatments were administered via gastric lavage for 7 days. After the treatments, blood samples were collected for testing the levels of total cholesterol (TC), triglycerides (TG), highdensity lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), malondialdehyde (MDA), superoxide dismutase (SOD), alanine aminotransferase (GPT), and aspartate aminotransferase (GOT); Western blotting was used to detect the expressions of the proteins involved in lipid metabolism in the liver tissues including ABCA1, ABCG8, LDLR, and SR-B1. Results Compared with the normal control group, the mice with Triton-WR1339-induced hyperlipidemia showed significantly increased levels of TC, TG, LDL, MDA, GPT, and GOT and lowered HDL-C levels and SOD activity (P<0.05). Treatments with fenofibrate and the 2 propolis at either low or high dose significantly reversed Triton-WR1339-induced changes in blood lipids (P<0.05), and the effects of propolis were more potent. Triton-WR1339 injection also significantly decreased the expressions levels of ABCA1, ABCG8, LDLR, and SR-B1 in the liver (P<0.05), and these changes were obviously reversed by treatments with fenofibrate and propolis (P<0.05), especially by the latter. Conclusion The lipid-lowering effects of propolis are mediated by improving lipid metabolism and regulating the expressions of lipid transport proteins in the liver tissue.

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更新日期/Last Update: 1900-01-01