[1]朱娜玲,许如,唐伟平,等.人持续性G病毒Ⅱ型在献血者和反复接受输血患者中的检测与意义[J].南方医科大学学报,2018,(07):842.
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人持续性G病毒Ⅱ型在献血者和反复接受输血患者中的检测与意义()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年07期
页码:
842
栏目:
出版日期:
2018-07-16

文章信息/Info

Title:
Detection of a novel human pegivirus HPgV-2 in healthy blood donors and recipients of multiple transfusions: implications for blood safety
作者:
朱娜玲许如唐伟平王海鹰万政伟吴学东付涌水唐时幸俞守义
关键词:
人持续性G病毒Ⅱ型献血者感染率血液安全
Keywords:
HPgV-2 blood donors viral infections blood safety
摘要:
目的确定我国献血者、反复接受输血患者HPgV-2感染率,阐明HPgV-2对血液安全的可能影响。方法运用血清学、反转 录聚合酶链反应(RT-PCR)等方法分别在1060份健康献血者、1402份HCV、500份HBV、570份反复接受输血者、248份血友病 患者共3780份血清标本中检测抗HPgV-2抗体和HPgV-2 RNA,对获得的HPgV-2 NS3片段和HPgV-2全基因组进行核酸序列 测定和分子进化分析。同时检测献血者、反复接受输血者和血友病患者血清丙型肝炎病毒(HCV)和乙型肝炎病毒(HBV)感染 情况辅助作为实验结果。结果(1)感染HCV(RNA+/Ab+)的献血者中抗HPgV-2抗体阳性率为1.21%(17/1402),核酸阳性率 为0.36%(5/1402),而其他几组人群中几乎未检出HPgV-2 病毒,这提示HPgV-2 感染可能主要与HCV密切相关(χ2=13.78,P= 0.004);(2)5株HPgV-2病毒保守区域NS3区同源性达97.11%,与国外分离毒株比较,其同源性为96.53%,未发现明显变异株。 结论健康献血者中HPgV-2的感染率很低,HPgV-2主要和HCV共感染,反复接受输血患者和血友病患者没有发现HPgV-2感 染,提示HPgV-2经输血传播不常见,可能对血液安全影响不明显。
Abstract:
Objective To investigate the prevalence of HPgV-2 in blood donors, transfusion recipients and hemophilia patients and its impact on blood safety. Methods Serum samples were collected from 1060 healthy blood donors, 1402 HCV-positive and 500 HBV- positive blood donors, 570 transfusion recipients and 248 hemophilia patients for screening anti-HPgV-2 antibodies, HPgV-2 RNA, anti-HCV and HBsAg/HBV-DNA using ELISA and RT-PCR. Phylogenetic analysis of near fulllength genome sequences and NS3 genes of pegiviruses and hepaciviruses were performed using MEGA software. Results Anti-HPgV-2 positivity and HPgV-2 RNA positivity were found in 1.21% (17/1402) and 0.36% (5/1402) of the blood donors infected with HCV (RNA+/Ab+), respectively, indicating a close correlation between HPgV-2 and HCV infection (χ2=13.78, P= 0.004). Anti-HPgV-2 antibody was hardly detected in the other populations. A nucleotide identity as high as 97.11% was found in the NS3 fragments among the 5 isolated HPgV-2 strains, which had a nucleotide identity of 96.53% with the reported strains isolated out of China. Conclusion The prevalence of HPgV-2 infection is rather low in healthy blood donors and transfusion recipients. Coinfection with HCV is common in HPgV-2 infection, and no evidence has now been available to support HPgV-2 transmission via blood transfusion, indicating that HPgV-2 may not pose a threat to blood safety.
更新日期/Last Update: 1900-01-01