[1]张佳露,胡国林,刘磊,等.沉默SLP-2 可抑制人宫颈癌细胞的迁移及侵袭能力[J].南方医科大学学报,2018,(07):812.
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沉默SLP-2 可抑制人宫颈癌细胞的迁移及侵袭能力()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年07期
页码:
812
栏目:
出版日期:
2018-07-16

文章信息/Info

Title:
Silencing of SLP-2 inhibits the migration and invasion of cervical cancer cells in vitro
作者:
张佳露胡国林刘磊陈伙娣李丹娟梁卫江
关键词:
宫颈癌迁移侵袭SLP-2上皮间质转化
Keywords:
cervical cancer migration invasion SLP-2 epithelial-mesenchymal transition
摘要:
目的研究沉默SLP-2对人宫颈癌细胞迁移及侵袭能力的影响及其作用机制。方法用siRNA转染人宫颈腺癌Hela细胞 及宫颈鳞癌Siha细胞作为沉默组,同时设立空白对照组及阴性对照组,转染48 h后,用Western blot检测SLP-2蛋白的表达量, 用划痕实验、Transwell 实验检测沉默SLP-2 对Hela 和Siha 细胞迁移能力的影响,用基质胶Transwell 实验检测沉默SLP-2 对 Hela 和Siha 细胞侵袭能力的影响,用Western blot 检测沉默SLP-2 后Hela 和Siha 细胞上皮间质转化标志分子E-cadherin、β- catenin、vimentin及上皮间质转化上游转录因子Twist蛋白表达量的变化。结果与对照组相比,Western blot结果显示沉默组 SLP-2蛋白的表达量明显下降;划痕实验显示沉默组的划痕面积愈合率明显下降(P<0.01);Transwell实验及基质胶Transwell实 验均显示沉默组穿过小室膜至下室的细胞数明显减少(P<0.01);Western blot结果显示沉默组上皮细胞标志分子E-cadherin及 β-catenin表达升高,而间质细胞标志分子vimentin表达下降,表明Hela细胞和Siha细胞的上皮间质转化受到抑制;Western blot 结果显示沉默组Twist蛋白的表达量也明显下调。结论沉默SLP-2可抑制人宫颈癌细胞上皮间质转化(EMT)的发生,使宫颈 癌细胞的迁移及侵袭能力下降,其作用机制可能与下调Twist蛋白的表达有关。
Abstract:
Objective To investigate the effect of SLP-2 silencing on the migration and invasion of human cervical cancer cells and explore the mechanism. Methods Small interfering RNA (siRNA) was used to knockdown the expression of SLP-2 in Hela cells and Siha cells. At 48 h after the transfection, the cells were examined for SLP-2 expression with Western blotting, and wound healing assay and Transwell assay were used to evaluate the changes in the cell migration; Matrigel Transwell assay was used to evaluate the changes in the invasion ability of the cells. The expressions of E-cadherin, β-catenin, vimentin and Twist in Hela and Siha cells following the transfection were detected with Western blotting. Results Compared with the control cells, siRNA transfection significantly lowered the expression of SLP-2 and suppressed the migration and invasion ability of Hela cells and Siha cells (P<0.01). Silencing SLP-2 induced obvious up-regulation of epithelial cell phenotype proteins E-cadherin and β-catenin, down- regulated the expression of interstitial cell phenotype protein vimentin, and lowered the expression of Twist in the cells. Conclusion Silencing SLP-2 via siRNA transfection can inhibit epithelial-mesenchymal transition of human cervical cancer cells and lower their migration and invasion abilities possibly in relation with downregulated expression of Twist.

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更新日期/Last Update: 1900-01-01