[1]姜婷婷,张雯英,向晓红,等.氯化锂通过AKT/GSK-3β阻滞近端肾小管上皮细胞周期在G2期[J].南方医科大学学报,2018,(05):541.
点击复制

氯化锂通过AKT/GSK-3β阻滞近端肾小管上皮细胞周期在G2期()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年05期
页码:
541
栏目:
出版日期:
2018-05-15

文章信息/Info

Title:
Lithium chloride arrests HK-2 cell cycle in G2 phase through AKT/GSK-3β signal pathway
作者:
姜婷婷张雯英向晓红束双双谢唯汤珣章俊
关键词:
氯化锂AKT/GSK-3βHK-2细胞G2期
Keywords:
lithium chloride AKT/GSK-3β HK-2 cells cell cycle
摘要:
目的探讨氯化锂(LiCl)是否影响近端肾小管上皮细胞(HK-2 细胞)的细胞周期及其信号通路。方法将不同浓度(5、 12.5、20、25 mmol/L)的LiCl作用于HK-2细胞不同时间(12、24、48、72 h),采用流式细胞术检测细胞周期变化,CCK-8测定HK-2 细胞的活力,Western blotting 检测细胞周期G2 期的关键蛋白Cyclin B1、CDK1 以及AKT/GSK-3β信号通路相关蛋白的表达 量。结果流式细胞术结果提示LiCl以时间-浓度依赖的方式使HK-2细胞的G2细胞周期比例逐渐增加(P<0.05),CCK-8结果 提示HK-2 细胞的活力随LiCl 作用的时间-浓度的增加而降低(P<0.05),Western blotting 显示Cyclin B1、CDK1、p-GSK-3β、β- catenin蛋白含量随着时间以及浓度的增加而增加,而AKT磷酸化水平随着时间及浓度的增加而减少(P<0.05)。结论LiCl可 能通过激活AKT/GSK-3β信号通路使HK-2细胞阻滞在G2期。
Abstract:
Objective To investigate the effect of lithium chloride (LiCl) on cell cycle of HK-2 cells and explore the possible pathways involved. Methods HK-2 cells were treated with LiCl at different concentrations (5, 12.5, 20, and 25 mmol/L) for 12, 24, 48, or 72 h, and the changes in cell cycle and viability were detected using flow cytometry and CCK-8 assay, respectively. Western blotting was used to analyze the changes in the expressions of cyclin B1 and CDK1 (the two G2 phase-related proteins) and those of AKT/GSK-3β signaling pathway-related proteins in the treated cells. Results LiCl treatment time- and concentration-dependently increased HK-2 cell percentage in G2 phase and decreased the cell vitality. The expressions of cyclin B1, CDK1, p-GSK-3β, and β-catenin increased and the expression of p-AKT decreased significantly in the cells as LiCl treatment time and concentration increased. Conclusion LiCl may cause HK-2 cell cycle arrest in G2 phase through activation of the AKT/GSK-3β signaling pathway.

相似文献/References:

[1]陈小乐,龚建平,徐发良.脂多糖预处理导致的糖原合成酶激酶-3抑制对肝糖原的影响和机制[J].南方医科大学学报,2014,(02):201.
[2]李理,覃永保,马刚,等.氯化锂复合磷酸钙骨水泥可促进大鼠胫骨骨缺损修复[J].南方医科大学学报,2016,(06):824.

更新日期/Last Update: 1900-01-01