[1]王瑜,王博士,惠旭,等.可诱导共刺激分子介导的Th17细胞极化对自发性高血压大鼠肾纤维化的影响[J].南方医科大学学报,2018,(05):534.
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可诱导共刺激分子介导的Th17细胞极化对自发性高血压大鼠肾纤维化的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年05期
页码:
534
栏目:
出版日期:
2018-05-15

文章信息/Info

Title:
Role of inducible costimulatory molecule-mediated Th17 cell polarization in renal fibrosis in spontaneously hypertensive rats
作者:
王瑜王博士惠旭乔君李委振孙楠
关键词:
可诱导共刺激分子Th17细胞自发性高血压大鼠肾纤维化
Keywords:
inducible costimulatory molecule Th17 cells spontaneously hypertensive rats renal fibrosis
摘要:
目的探讨可诱导共刺激分子(ICOS)介导的Th17细胞极化在原发性高血压肾损害中的作用。方法4周龄的自发性高血 压大鼠(SHR)随机分为SHR-C组及SHR-I组,分别用0.01 mol/L磷酸缓冲盐溶液(PBS)、ICOS阻断型单克隆抗体干预2周。采 用无创尾动脉血压测量仪动态监测大鼠的血压。采用流式细胞技术分析大鼠脾淋巴细胞中Th17细胞的频数变化。RT-PCR法 动态检测大鼠肾脏中IL-17AmRNA的表达水平。采用免疫组化技术及ELISA法动态检测大鼠肾脏及血浆中IL-17A和TGF-β 1的表达水平。采用Masson染色检测大鼠肾脏病理改变情况。结果SHR-C组大鼠从第10周始血压显著高于同期的SHR-I组 大鼠(P<0.05或P<0.01)。从6周始SHR-C组的大鼠Th17细胞频数显著高于同期SHR-I组(P<0.05)。SHR-C组的大鼠肾脏中 IL-17A mRNA表达亦显著高于同期SHR-I 组(P<0.05),且其血浆和肾脏中IL-17A、TGF-β1 的表达显著高于同期SHR-I 组 (P<0.05)。与SHR-C组相比,SHR-I组的大鼠肾脏纤维化程度降低,在30周两者差异具有显著性(P<0.05)。结论由ICOS介 导的Th17细胞极化在高血压导致的肾纤维化中起重要作用。
Abstract:
Objective To explore the role of inducible costimulatory molecule (ICOS) signaling pathway- mediated Th17 cells polarization in renal damage in essential hypertension. Methods Four-week-old spontaneously hypertensive rats (SHR) were randomly divided into control (SHR-C) group and intervention (SHR-I) group and subjected to intraperitoneal injections of PBS and ICOS monoclonal antibody for 2 weeks, respectively. Blood pressure of the rats was monitored using noninvasive tail artery blood pressure measuring instrument. The percentage of Th17 cells in the splenocytes was analyzed using flow cytometry, and the expression levels of IL-17A mRNA in the rat’s kidneys were detected using RT-PCR. The levels of IL-17A and TGF-β1 in the plasma and kidneys were dynamically detected using ELISA and immunohistochemistry, respectively. Renal pathological changes in the rats were detected using Masson staining. Results At the age of 10 and 30 weeks, the rats in SHR-C group had a significantly higher blood pressure than those in SHR-I group (P<0.05 or 0.01). In rats in SHR-C group, Th17 cells percentage in the splenocytes and IL-17A mRNA level in the kidney was significantly higher than those in SHR-I group from the age of 6 weeks (P<0.05). The expressions of IL-17A and TGF-β1 in the plasma and kidney were significantly higher in SHR-C group than that in SHR-I group at 6 weeks (P<0.05). Compared with those in SHR-C group, the rats in SHR-I group showed significant alleviation of renal fibrosis from the age of 30 weeks (P<0.05). Conclusion The ICOS signaling pathway-mediated Th17 cells polarization plays an important role in renal fibrosis in hypertensive rats.

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[2]顾冬生,熊露,韩艳萍,等.坏死细胞逆转脓毒症小鼠Treg/Th17比例的失衡[J].南方医科大学学报,2014,(07):956.
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更新日期/Last Update: 1900-01-01