[1]周鑫,张磊,扶世杰,等.少阳主骨方介导p19Arf-p53-p21Cip1信号通路调控食蟹猴关节软骨退变的机制[J].南方医科大学学报,2018,(03):346.
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少阳主骨方介导p19Arf-p53-p21Cip1信号通路调控食蟹猴关节软骨退变的机制()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年03期
页码:
346
栏目:
出版日期:
2018-04-03

文章信息/Info

Title:
Shaoyangzhugu Formula regulates p19Arf-p53-p21Cip1 signaling pathway to ameliorate cartilage degeneration in aged cynomolgus monkeys with knee osteoarthritis
作者:
周鑫张磊扶世杰刘刚郭晓光易刚刘洋汪国友
关键词:
少阳主骨食蟹猴KOA模型细胞衰老p19Arf-p53-p21Cip1信号通路
Keywords:
Shaoyangzhugu Formula cynomolgus monkeys knee osteoarthritis cell senescence p19Arf-p53-p21Cip1 signaling pathway
摘要:
目的根据“少阳主骨”理论,研究少阳主骨方介导p19Arf-p53-p21Cip1信号通路调控关节软骨退变的机制。方法通过X线 选取13只膝关节退变的老年食蟹猴,随机选取1只进行病理学观察,其余食蟹猴随机分为少阳主骨方组、氨糖美辛组、生理盐水 组,每组4只,连续灌胃8周后处死所有食蟹猴,取关节软骨进行病理学观察,RT-qPCR、Western blot检测关节软骨中p19Arf、p53、 p21Cip1基因与蛋白的表达。结果老年食蟹猴KOA模型关节软骨符合KOA病理变化,3 组食蟹猴Mankin 评分少阳主骨方组 7.38±0.52 分、氨糖美辛组7.88±0.83 分、生理盐水组8.38±0.74 分,少阳主骨方组与生理盐水组间具有统计学差异(P<0.05); p19Arf、p53、p21Cip1基因与蛋白的表达少阳主骨方组<氨糖美辛组><生理盐水组,具有统计学差异(p><0.05)。结论少阳主骨方可 以延缓关节软骨退变,调控p19arf-p53-p21cip1表达。="">
Abstract:
Objective To study the effect of Shaoyangzhugu (SYZG) Formula (a formula consisting of 9 traditional Chinese drugs) in delaying the degeneration of articular cartilage and the role p19Arf-p53-p21Cip1 signaling pathway in mediating this effect. Method Thirteen aged cynomolgus monkeys with degenerative knee joints were selected based on X-ray findings, and one of them was randomly selected for pathological observation. The other monkeys were randomized equally into SYZG Formula group (treated with SYZG decoction), ammonia moxime group and saline group. All the monkeys were sacrificed after 8 weeks of treatment with intragastric administration of the drugs or saline. The pathology in the knee joint articular cartilage was observed and the mRNA and protein expressions of p19Arf, p53, and p21Cip1 in the articular cartilage were detected using RT-qPCR and Western blotting. Results The pathological findings of the articular cartilage in old cynomolgus monkeys were consistent with the characteristics of knee osteoarthritis (KOA). Mankin scores of the cynomolgus monkeys were 7.38±0.52 in SYZG Formula group, 7.88±0.83 in ammonia moxime group, and 8.38±0.74 in saline group, showing a significant difference between SYZG Formula group and saline group (P<0.05). The expressions of p19Arf, p53, and p21Cip1 were the lowest in SYZG Formula group and the highest in saline group with significant differences among the 3 groups (P<0.05). Conclusion SYZG Formula can delay chondrocyte senescence by regulating p19Arf- p53- p21Cip1 signaling pathway to delay articular cartilage degeneration in aged cynomolgus monkeys.
更新日期/Last Update: 1900-01-01