[1]尹丝璐,胡坚,李维娜,等.精液细菌感染对精液参数及细菌耐药性的影响—附74376例男性不育症患者分析[J].南方医科大学学报,2018,(01):89.
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精液细菌感染对精液参数及细菌耐药性的影响—附74376例男性不育症患者分析()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年01期
页码:
89
栏目:
出版日期:
2018-01-20

文章信息/Info

Title:
Effect of semen bacterial infection on semen parameters and analysis of drug resistance in 74376 male infertility patients
作者:
尹丝璐胡坚李维娜石林刘刚
关键词:
精液质量细菌感染药敏分析
Keywords:
semen quality bacterial infection drug sensitivity analysis
摘要:
目的男性不育症患者精液细菌感染对精液参数的影响和耐药分析。方法收集2016年4月~2017年4月在本院就诊的 74376例男性不育患者的精液标本,按照细菌培养结果分为感染组和非感染组,比较两组不育症类型和精液参数的差异。对检 出菌的种类进行统计,及药敏结果进行分析。结果精液细菌感染阳性率为1.38%,其中精液正常组阳性率为1.41%,弱精症组 感染阳性率为1.55%,少精症感染阳性率为1.18%,少弱精症感染阳性率为1.57%,无精症感染阳性率为0.17%,无精症组阳性率 低于其他组;细菌感染主要影响精子活力(P<0.05)、精子密度和前向精子比例(P<0.01);细菌感染主要种类为:大肠埃希菌占 63.59%,肺炎克雷伯菌肺炎亚种占19.80%和奇异变形杆菌占13.22%;药敏结果显示大肠埃希菌、肺炎克雷伯菌肺炎亚种、克氏 柠檬酸杆菌对阿莫西林明显耐药,铜绿假单胞菌对复方新诺明、氨苄西林/舒巴坦明显耐药,金黄色葡萄球菌对青霉素明显耐 药。结论不育患者精液细菌感染率较低,细菌感染对精子参与受精过程影响较大,可引起精子活力降低,密度减少。感染的细 菌种类中大肠杆菌所占比例最高;除金黄色葡萄球菌之外,其他菌种对亚胺培南,美洛培南类药物敏感。
Abstract:
Objective To investigate the effect of semen bacterial infection on semen parameters in male infertility patients and analyze of the drug resistance profile of the bacterial isolates. Methods According to the results of bacterial culture, the semen samples of 74376 infertile men collected between April, 2016 and April, 2017 were divided into infection group and noninfection group, and the infertility type and semen parameters were compared between the two groups. The bacterial species and the drug sensitivity of the isolates were analyzed. Results Bacterial infections were detected in 1.38% of the total semen samples collected. The positivity rate of semen bacterial infection was 1.41% in normal semen group, 1.55% in asthenospermia group, 1.18% in oligospermia group, 1.57% in asthenospermia/ oligospermia group, and 0.17% in azoospermia group. The positivity rate was lower in azoospermia group than in the other groups. Bacterial infection mainly affected sperm motility (P< 0.05), sperm density and forward motile sperm ratio (P<0.01). The most common bacterial species causing the infections included, in the descending order of frequencies, Escherichia coli (63.59%), Klebsiella pneumoniae subspecies (19.80%) and Proteus mirabilis (13.22%). Drug susceptibility tests showed that the isolates of Escherichiacoli, Pneumonia Klebsiella pneumonia subspecies and Citrobacter koseri were commonly resistant to amoxicillin; Pseudomonas aeruginosa isolates were resistant to Bactrim and ampicillin/sulbactam; Staphylococcus aureus isolates were resistant to penicillin. Conclusion Male infertile patients have a low bacterial infection rate in the semen. Bacterial infection severely affects the sperm fertilization process by causing impairment of sperm motility and lowered sperm density. Escherichia coli is the most common pathogenic bacterium for the semen infection. With the exception of Staphylococcus aureus, the other bacterial strains isolated were found to be sensitive to imipenem and meropenem.
更新日期/Last Update: 1900-01-01