[1]廖哲霆,邢祯全,陈宇璠,等.关节腔注射抗坏血酸/氯化铁可延缓骨性关节炎大鼠软骨退变[J].南方医科大学学报,2018,(01):62.
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关节腔注射抗坏血酸/氯化铁可延缓骨性关节炎大鼠软骨退变()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年01期
页码:
62
栏目:
出版日期:
2018-01-20

文章信息/Info

Title:
Intra-articular injection of ascorbic acid/ferric chloride relieves cartilage degradation in rats with osteoarthritis
作者:
廖哲霆邢祯全陈宇璠邓仲豪吴德胜赵亮
关键词:
骨性关节炎抗坏血酸氯化铁关节腔注射芬顿反应潜伏TGFβ1
Keywords:
osteoarthritis ascorbic acid ferric chloride intra-articular injection fenton reaction latent TGFβ1
摘要:
目的探索关节腔注射抗坏血酸-氯化铁混合液(AA/FeCl3)对骨性关节炎大鼠关节退变的影响。方法将30只成年雄性骨 性关节炎大鼠随机等分为两组,自第3周起分别予每周1次关节腔注射生理盐水(对照组)或AA/FeCl(3 实验组)。分别于第6、9、 12周随机处死每组5只大鼠,使用X线评估关节内骨改变,标本肉眼观、番红/固绿染色和OARSI评分体系评估关节软骨退变, Ⅱ型胶原免疫组化观察细胞外基质变化。结果9周时仅对照组X线下见关节面不平整,12周时对照组关节线消失,实验组出 现轻微关节面不平;对照组9周标本肉眼可见明显软骨溃疡,12周见大面积软骨缺损,实验组9周软骨表面粗糙,12周见小范围 软骨溃疡;番红/固绿及OARSI评分示第9、12周实验组软骨退变显著轻于对照组(9周对照组vs实验组:18.67±0.67 vs 12.17± 2.75;12 周:20.11±1.84 vs 13.77±0.40,P<0.05),第6、9 周实验组软骨层Ⅱ型胶原含量显著高于对照组(6 周对照组vs 实验组: 0.36±0.039 vs 0.49±0.029;9周:0.25±0.041 vs 0.38±0.040,P<0.05)。结论早期关节腔注射抗坏血酸-氯化铁混合液能有效延缓 骨性关节炎大鼠的软骨退变。
Abstract:
Objective To assess the effect of ascorbic acid/ferric chloride (AA/FeCl3) in attenuating cartilage damage in rats with osteoarthritis. Methods Thirty adult male Wistar rats with surgically induced osteoarthritis were randomized into 2 groups for treatment with intra-articular injection of saline (control group) or AA/FeCl3 mixture (AA group) once a week starting from the third week after the operation. At 6, 9, and 12 weeks after the operation, 5 rats from each group were sacrificed for observing subchondral bone changes on X-ray films and evaluation of cartilage degeneration in the right knee joints using safranin-O/Fast green staining and a modified OARSI scoring system. The degradation of the cartilage matrix was observed by immunohistochemical staining for type II collagen. Results X-ray examination in saline control group revealed the presence of osteophytes and narrowing of the joint space at 9 weeks, and the joint line disappeared at 12 weeks after the surgery; only slight irregularity of the articular surface was observed in the AA group at 9 and 12 weeks. OARSI scores were significantly lower in AA group than in the control group at 9 weeks (18.67±0.67 vs 12.17±2.75; P<0.05) and 12 weeks (20.11±1.84 vs 13.77± 0.40; P<0.05) but not at 6 weeks after the surgery. The content of type 2 collagen in AA group was significantly higher than that in the control group at 6 weeks (0.36±0.039 vs 0.49±0.029; P<0.05) and 9 weeks after the surgery (0.25±0.041 vs 0.38±0.040; P< 0.05). Conclusions Early intra-articular injection of AA/FeCl3 can effectively delay the progression of post-traumatic osteoarthritis in rats.

相似文献/References:

[1]林志春,翟磊,陈燕萍,等.重T2GRE多回波序列在膝关节软骨病变中的应用[J].南方医科大学学报,2011,(06):1095.
[2]陈聪,马愔花,谭小义,等.冠状位髁间窝宽度指数与骨关节炎的关系[J].南方医科大学学报,2015,(10):1384.
[3]黄志会,宋兵,陈宇璠,等.抗坏血酸/聚已酸内酯生物材料修复新西兰白兔软骨缺损[J].南方医科大学学报,2017,(05):607.

更新日期/Last Update: 1900-01-01