[1]罗东,罗月苹,刘宝茹,等.新西兰兔再生障碍性贫血模型的建立[J].南方医科大学学报,2017,(12):1660.
点击复制

新西兰兔再生障碍性贫血模型的建立()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年12期
页码:
1660
栏目:
出版日期:
2017-12-20

文章信息/Info

Title:
Establishment of New Zealand rabbit models of aplastic anemia
作者:
罗东罗月苹刘宝茹梁丹丹蒋璟玮汪威陈俊林王嫣陈文直
关键词:
再生障碍性贫血环磷酰胺正交试验动物模型
Keywords:
aplastic anemia benzene cyclophosphamide orthogonal test animal models
摘要:
目的正交设计筛选苯和环磷酰胺联合使用建立新西兰兔再生障碍性贫血模型的适宜剂量。方法新西兰兔,采用正交试 验设计,应用L9(34)正交表对苯的剂量(A)、环磷酰胺的剂量(B)、苯的注射次数(C)、环磷酰胺的注射次数(D)四个因素及它们 各自不同的3个水平在建立新西兰兔再生障碍性贫血模型,并最终从9个实验组中优选出建模的较优方案。给药方法为先背部 皮下注射苯,隔日1次,再耳缘静脉注射环磷酰胺,每天注射,均按照规定次数注射。每6 d检测1次血常规,于建模前、建模后 36d取小段股骨进行骨髓组织学检查,观察变化。结果将9组白细胞、红细胞、血小板计数进行对比分析,4~9组新西兰兔再障 模型建立成功,第7、8、9组与其他组日均下降速率之差有统计学意义(P<0.05),其中第7组骨髓切片显示骨髓增生低下,造血组 织减少,巨核细胞减少或消失,脂肪细胞增多。随访发现,第7组骨髓抑制一直存在,与该病临床特点相符合。结论第7组采用 苯1.5 mL/kg,8次/d,环磷酰胺10 mg/kg,4次/d,这一组合剂量建立的新西兰兔AA模型建模周期短、且模型稳定,是一种可广泛 应用于动物实验的建模方法。
Abstract:
Objective To screen for the optimal dose of benzene and cyclophosphamide using an orthogonal design for establishment of New Zealand rabbit models of aplastic anemia. Methods Following an orthogonal experimental design, the effects of 3 levels of 4 factors, namely the dose of benzene (A), the dose of cyclophosphamide (B), the number of benzene injections (C), and the number of cyclophosphamide injections (D) were tested in the establishment of New Zealand rabbit models of aplastic anemia using a L9 (34) orthogonal table, and the optimal protocol for the model establishment was selected from the 9 experimental groups. Each rabbit received subcutaneous injection of benzene on the back every other day, followed by daily cyclophosphamide injection via the ear vein for prescribed times. The blood routine was examined every 6 days, and before modeling and at 36 days after modeling, a small sample of the femoral bone was collected for bone marrow histopathological examination. Results Comparison of the white blood cell, erythrocyte and platelet counts among the 9 groups showed successful modeling in Groups 4-9, and daily mean reduction rates of the cell counts in Groups 7, 8, and 9 differed significantly from those in the other groups (P<0.05). In Group 7, bone marrow sections showed low myelodysplasia, reduced hematopoietic tissue, reduced or even absence of megakaryocytes, and increased fat cells. Further observation found that the rabbits in Group 7 had sustained bone marrow suppression, consistent with the clinical characteristics of the disease. Conclusion Stable models of aplastic anemia can be established efficiently in New Zealand rabbits by a combination of 8 subcutaneous injections of benzene at 1.5 mL/kg and 4 intravenous injections of cyclophosphamide at 10 mg/kg.

相似文献/References:

[1]邓伟,席亚明,曹长青.肝炎相关再生障碍性贫血8例临床分析[J].南方医科大学学报,2011,(08):1443.
[2]杨楠,马肖容,张卉,等.猪抗人淋巴细胞球蛋白与兔抗人胸腺细胞球蛋白治疗重型再生障碍性贫血的疗效比较[J].南方医科大学学报,2016,(03):303.

更新日期/Last Update: 1900-01-01