[1]王静,谭伟江,李想,等.高频超声心动图评价心梗小鼠左室整体功能和节段性室壁运动异常[J].南方医科大学学报,2017,(08):1014.
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高频超声心动图评价心梗小鼠左室整体功能和节段性室壁运动异常()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年08期
页码:
1014
栏目:
出版日期:
2017-08-06

文章信息/Info

Title:
High-frequency echocardiography for assessment of regional wall motion abnormality
and cardiac function in mice with myocardial infarction
作者:
王静谭伟江李想张贵平黄家园陈小红雷迁杨丰华黄韧
关键词:
高频超声心动图心梗模型左室收缩功能左室舒张功能局部室壁运动指数
Keywords:
high-frequency echocardiographymyocardial infarction ventricular systolic functionventricular diastolic function regional ventricular wallmotion index
摘要:
目的通过建立小鼠心梗模型,探讨应用超高分辨率超声系统评价心梗小鼠心脏结构及心功能方面的应用价值。方法25
只雄性C57BL/6小鼠随机分为2组,即开胸结扎左冠状动脉前降支建立心梗模型组(n=15)和仅在相同结扎位置穿针,不结扎的
方法建立假手术组(n=10)。术前3 d和术后1周采用二维、M型、脉冲多普勒(PWD)、组织多普勒(TDI)和EKVTM超声技术对心
梗小鼠心脏形态结构、室壁运动以及心功能进行评价。术后1周,取小鼠心脏进行HE染色并观察心肌组织结构。结果二维超
声与HE色染均显示,心梗模型组心肌有明显的梗死区;心梗模型组与假手术组比较,心室明显扩大,室壁明显变薄,心室收缩功
能与舒张功能均显著下降(P<0.05);局部室壁运动受阻严重,心室发生重构。结论采用2D超声心动图联合M型、PWD、TDI以
及EKVTM超声心动图观察小鼠心梗模型能够更精准敏感地判断心肌梗死位置和梗死程度,为临床心肌梗死诊断、治疗和预后评
估提供了参考价值。
Abstract:
Objective To evaluate the value of high-frequency echocardiography in assessing cardiac structure and function in a
mouse model of myocardial infarction. Methods Twenty-five C57BL/6 mice were randomly divided into sham-operated group
(n=10) and myocardial infarction model group (n=15) established by ligation of the left anterior descending artery. The cardiac
structure, regional wall motion and cardiac function of mice were examined with pulsed wave Doppler (PWD), tissue Doppler
imaging (TDI), EKV and M-mode echocardiography 3 days before and at 1 week after the operation. The histological changes
and myocardial structure of the heart were observed at 1 week after the operation. Results High-frequency echocardiography
and HE staining detected obvious myocardial infarction in the mice in the model group. Compared with the sham-operated
mice, the mice with myocardial infarction showed significant left ventricular expansion, obvious thinning of the ventricular
wall, and significantly decreased ventricular systolic
function and diastolic function with regional wall motion
abnormality and ventricular remodeling. Conclusions
2D-type echocardiography combined with M-mode,
PWD, TDI and EKVTM for allows accurate and sensitive
detection of the loci and severity of myocardial infarction
to provide important evidence for clinical diagnosis and
treatment of myocardial infarction.
更新日期/Last Update: 1900-01-01