[1]黄昊,汤红峰,陈颖,等.强脉冲光对红毛癣菌的体外抑制作用[J].南方医科大学学报,2017,(06):853.
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强脉冲光对红毛癣菌的体外抑制作用()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年06期
页码:
853
栏目:
出版日期:
2017-06-20

文章信息/Info

Title:
Effect of intense pulsed light on Trichophyton rubrum growth in vitro
作者:
黄昊汤红峰陈颖郑秀芬胡勇王瑞华黄美玲
关键词:
红色毛癣菌强脉冲光活性氧氧化应激
Keywords:
Trichophyton rubrum intense pulsed light reactive oxygen species oxidative stress
摘要:
目的探讨强脉冲光420 nm滤光片在体外对红色毛癣菌的抑制作用及其机制。方法实验所用红色毛癣菌落及菌悬液各 分为处理组(强脉冲光照射)和对照组(未照射),在照射前、照射后第2天、第3天,拍摄各组菌落,使用Photoshop cx7软件以像素 计算菌落的面积观察菌落生长曲线变化,以及在照射6 h后,通过噻唑蓝检测真菌菌悬液的活力;2’,7’-二氯荧光黄双乙酸盐荧光 探针检测各组真菌内活性氧变化,硫代巴比妥酸法检测各组真菌丙二醛含量改变。结果IPL420 nm对红色毛癣菌具有损伤作 用,其最适有效治疗参数为12 J/cm2 12脉冲,处理后6 h后菌株活力下降约30%(P<0.05),而处理后平板菌落停止生长,生长曲 线趋于直线;同时IPL处理后真菌内活性氧水平升高(P<0.05),伴随丙二醛含量上升(P<0.05)。结论IPL420 nm可在体外通过 诱导红色毛癣菌内产生氧化应激,导致真菌损伤和死亡。
Abstract:
Objective To investigate the inhibitory effect of 420 nm intense pulsed light on Trichophyton rubrum growth in vitro and explore the mechanism. Methods The fungal conidia were divided into treatment group with intense pulse light irradiation and control group without irradiation. The surface areas of the fungal colonies were photographed before irradiation and on the 2nd and 3rd days after irradiation to observe the changes in fungal growth. The viability of the fungus in suspension was detected at 6 h after irradiation using MTT assay. The intracellular reactive oxygen species (ROS) level in the fungus was determined using DCFH-DA fluorescent probe, and the MDA content was detected using TBA method. Results Intense pulse light (420 nm) irradiation caused obvious injuries in Trichophyton rubrum with the optimal effective light dose of 12 J/cm2 in 12 pulses. At 6 h after the irradiation, the fungus in suspension showed a 30% reduction of viability (P<0.05), and the fungal colonies showed obvious growth arrest without further expansion. Compared to the control group, the irradiated fungus showed significant increases in ROS level and MDA content (P<0.05). Conclusion Intense pulse light (420 nm) irradiation can induce oxidative stress in Trichophyton rubrum to lead to fungal injuries and death.

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[1]王明利,柳大烈,袁强.强脉冲光对皮肤热休克蛋白70表达的影响[J].南方医科大学学报,2005,(01):109.
 WANG Ming-li,LIU Da-lie,YUAN Qiang.Effect of intense pulsed light on heat shock protein 70 expression in skin[J].,2005,(06):109.

更新日期/Last Update: 1900-01-01