[1]邹琼,高艳彬,金辉,等.小鼠烧伤早期免疫细胞基因表达谱分析及靶基因的筛选[J].南方医科大学学报,2017,(06):767.
点击复制

小鼠烧伤早期免疫细胞基因表达谱分析及靶基因的筛选()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年06期
页码:
767
栏目:
出版日期:
2017-06-20

文章信息/Info

Title:
Screening of biomarkers related with leukocyte responses early after burn injury in mice by differential gene expression profiling
作者:
邹琼高艳彬金辉卢志阳史鹏伟杨磊
关键词:
烧伤免疫系统过程差异表达基因蛋白互作网络
Keywords:
post-burn injury immune system process differentially expressed genes protein-protein interaction network
摘要:
目的利用生物信息学方法对小鼠烧伤早期免疫细胞差异基因表达谱进行分析并检测,筛选相关差异基因,以期找到潜在 的诊断及治疗靶标。方法从GEO数据库中下载GSE7404数据集,通过配对样本t检验、倍比法及GO功能富集分析筛选差异 表达基因,应用STRING数据库及Cytoscape 软件构建蛋白互作网络筛选相关基因靶标,运用实时荧光定量PCR及Western blotting进行试验验证。结果在烧伤后第1天,有1825个基因被选出,其中上调基因658个,下调基因1167个。运用STRING 数据库及Cytoscape软件构建蛋白互作网络分析,发现Stat1、Cdk1、Cd19、Lck及Jun基因在烧伤后免疫系统功能变化过程中可能 起到重要作用。实时荧光定量PCR及Western blotting检测显示Stat1、Cdk1和Jun基因表达情况与分析结果相一致。结论通 过对小鼠烧伤后早期全血游离白细胞基因芯片分析我们发现Stat1、Cdk1和Jun基因在烧伤早期免疫细胞功能变化的过程中发 挥重要的作用,可能是烧伤早期诊断及治疗的潜在的生物学靶标。
Abstract:
Objective To screen the genes related with leukocyte responses in mice early after burn injury by bioinformatic analysis of the gene expression profiling data. Methods The gene expression profiles were obtained from GEO (GSE7404, Mouse musculus, 25% TBSA, full-thickness) database. T test, fold changes and GO functional enrichment analysis were used to identify the differentially expressed genes (DEGs) related to leukocyte responses to burns; the interacting genes were transferred to STRING to construct the protein-protein interaction (PPI) network. Biological annotation of the sub-networks was executed using the software Cytoscape. Real-time PCR and Western blotting were used to verify the DEGs in mice. Results In mice at 1 day post-burn, a total of 658 genes were up-regulated and 1167 were down-regulated. PPI network and module analysis suggested that some of the genes (Stat1, Cdk1, Cd19, Lck and Jun) may play critical roles in the PPI network post-burn. Real-time PCR and Western blotting results in mice were consistent with those of bioinformatic analysis of Stat1, Cdk1 and Jun. Conclusion Stat1, Cdk1 and Jun might be critical players in the development of leukocyte response in mice early after burn injury. Our finding provides new insights into the pathogenesis of leukocyte response to burn injury and identifies several biomarkers as potential targets for burn treatment.

相似文献/References:

[1]陈云辉,王甲汉,李志清,等.Exendin-4对严重烫伤大鼠早期心肌细胞凋亡的影响[J].南方医科大学学报,2011,(06):1101.
[2]周岳平,张春新,陈贵彬,等.磨痂术配合生物敷料A在四肢深II度烧伤中的应用[J].南方医科大学学报,2006,(05):680.
 ZHOU Yue-ping,ZHANG Chun-xin,CHEN Gui-bing Department of Burn Surgery,et al.Clinical evaluation of eschar grinding and biological dressing A for treatment of deep partial-thickness burn wound on the extremities[J].,2006,(06):680.
[3]陈华德,赖文,郑少逸,等.层流洁净技术在烧伤治疗中的应用[J].南方医科大学学报,2005,(12):1589.
 CHEN Hua-de,LAI Wen,ZHENG Shao-yi,et al.Application of laminar air flow techniques in burn treatment[J].,2005,(06):1589.
[4]邱学文,王甲汉,方小文,等.鸸鹋油对烫伤大鼠创面的抗炎及促愈合作用[J].南方医科大学学报,2005,(04):407.
 QIU Xue-wen,WANG Jia-han,FANG Xiao-wen,et al.Anti-inframnatory activity and healing-promoting effects of topical application of emu oil on wound in scalded rats[J].,2005,(06):407.
[5]陈波,郭晓华,王述昀,等.肌球蛋白轻链激酶在烧伤大鼠血清引起的内皮细胞骨架改变过程中的作用[J].南方医科大学学报,2004,(05):481.
 CHEN Bo,GUO Xiao-hua,WANG Shu-yun,et al.Myosin light-chain kinase contributes to short-term endothelial cell cytoskeletal alteration induced by serum from burned rats[J].,2004,(06):481.
[6]邱学文,王甲汉,李志清.特大面积烧伤伴重度吸入性损伤的救治[J].南方医科大学学报,2004,(05):597.
 QIU Xue-wen,WANG Jia-han,LI Zhi-qing.Management of extensive burn accompanying severe inhalation injury[J].,2004,(06):597.
[7]李亚洁,张立颖,罗炳德,等.湿热环境下Ⅱ度烫伤大鼠施行冷却疗法后体核温度的改变[J].南方医科大学学报,2004,(02):152.
 LI Ya-jie,ZHANG Li-ying,LUO Bing-de,et al.Rectal temperature changes of Wistar rats with second-degree scald burn in hot and humid environment following immediate cooling therapy[J].,2004,(06):152.
[8]石胜军,肖能坎,刘洪.去细胞异体真皮与自体皮片复合移植的临床研究[J].南方医科大学学报,2004,(11):1306.
 SHI Sheng-jun,XIAO Neng-kan,LIU Hong.Clinical application of composite skin graft consisting of acellular allogenic dermis and autologous skin[J].,2004,(06):1306.
[9]梁蓉,荣新洲,张涛,等.甘露醇对新西兰兔严重烧伤早期血清TNF-α和IL-6的影响及其脏器功能的保护机制[J].南方医科大学学报,2013,(04):598.
[10]徐沛,王甲汉,史鹏伟,等.兔烫伤合并海水浸泡后加重肠道损伤的机制[J].南方医科大学学报,2014,(06):880.

更新日期/Last Update: 1900-01-01