[1]王好为,段志娇,胡莎莎,等.cAMP依赖型蛋白激酶抑制剂β在结直肠癌中的表达及其临床意义[J].南方医科大学学报,2017,(06):744.
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cAMP依赖型蛋白激酶抑制剂β在结直肠癌中的表达及其临床意义()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年06期
页码:
744
栏目:
出版日期:
2017-06-20

文章信息/Info

Title:
Expression of cAMP-dependent protein kinase inhibitor beta in colorectal carcinoma and its clinical significance
作者:
王好为段志娇胡莎莎王爽
关键词:
结直肠癌cAMP依赖型蛋白激酶抑制剂β免疫组织化学预后分期
Keywords:
colorectal carcinoma cAMP-dependent protein kinase inhibitor beta immunohistochemistry prognosis stage
摘要:
目的探讨结直肠癌组织中cAMP依赖型蛋白激酶抑制剂β(PKIB)的表达水平及其与结直肠癌患者临床病理因素之间的 关系。方法利用实时荧光定量PCR法检测34对结直肠癌组织和配对癌旁正常组织中PKIB的表达水平,应用免疫组织化学法 对72例结直肠癌组织中PKIB的表达水平进行检测,并分析PKIB表达的临床意义。结果在结直肠癌组织中PKIB mRNA及蛋 白的表达水平均明显高于配对癌旁正常组织(P<0.0001)。PKIB蛋白的表达水平与结直肠癌患者T分期正相关(P=0.038),而 与患者的年龄、性别、肿瘤大小、发病部位以及淋巴结转移(N分期)、远隔器官转移(M分期)无明显相关性(P>0.05)。高表达 PKIB的结直肠癌患者生存时间明显短于低表达的患者(70.532±6.190 vs 93.500±5.847,P=0.023)。结论PKIB在结直肠癌组织 中高表达,其高表达与结直肠癌患者T分期及预后不良呈正相关,提示PKIB可能在结直肠癌演进中发挥了重要作用并有望成 为一种新的结直肠癌预后判断标志物。
Abstract:
Objective To investigate the expression of cAMP-dependent protein kinase inhibitor beta (PKIB) in colorectal carcinoma (CRC) and its association with the clinicopathological factors of the patients. Methods The expression of PKIB mRNA was detected with quantitative real-time PCR in 34 CRC tissues and paired adjacent tissues. Immunohistochemistry was used to detect the expression of PKIB protein in 72 CRC tissue specimens, and the relationship between PKIB protein expression and the clinicopathological features of the patients was analyzed. Results The expression of PKIB mRNA was significantly higher in CRC tissues than in the paired asjacent tissues (P<0.0001). The expression of PKIB protein in CRC patients was closely related with tumor infiltration (T stage) (P=0.038) but not with age, gender, tumor size, location, lymph node metastasis (N stage) or distant metastasis (M stage) (P>0.05). The survival time of patients with high PKIB expressions was significantly shorter than that of patients with low PKIB expressions (70.532 ± 6.190 vs 93.500 ± 5.847 months, P=0.023). Conclusion A high expression of PKIB in CRC is positively correlated with tumor infiltration (T stage) and a poor prognosis, suggesting an important role of PKIB in the development of CRC and its value as an indicator for prognostic evaluation of CRC patients.

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更新日期/Last Update: 1900-01-01