[1]孙茫,沈炼桔,刘阳,等.低氧环境下丙泊酚可增加PC12细胞的凋亡[J].南方医科大学学报,2017,(02):216.
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低氧环境下丙泊酚可增加PC12细胞的凋亡()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年02期
页码:
216
栏目:
出版日期:
2017-01-22

文章信息/Info

Title:
Effect of propofol on apoptosis of PC12 cells under hypoxic condition and the mechanism
作者:
孙茫沈炼桔刘阳于晴龙春兰李上莹莹涂生芬
关键词:
丙泊酚低氧氧化应激PC12凋亡
Keywords:
propofol hypoxia oxidative stress PC12 cells apoptosis
摘要:
目的探讨低氧环境下丙泊酚对PC12细胞株凋亡的影响及机制。方法将PC12细胞接种于培养板中,采用随机数字表 法,将其随机分为6组:低氧对照组(CH组)、空气对照组(CA组)和氧气对照组(CO 组),对照组加入脂肪乳浓度10 μmol/L。丙 泊酚低氧组(PH 组)、丙泊酚空气组(PA组)和丙泊酚氧气组(PO组),丙泊酚浓度为10 μmol/L。药物处理完毕后分别放入低氧 (5% O2),空气和氧气(35% O2)环境的细胞培养箱培养,8 h后流式检测细胞凋亡情况,检测细胞内ROS水平和细胞SOD酶活 力。结果与CA组比较,PA组细胞凋亡增加,活性氧簇(ROS)升高,超氧化物歧化酶(SOD)活力增强;与CH组比较,PH组细胞 凋亡增加,ROS升高,SOD活力增强;与CO组比较,PO组细胞凋亡增加,ROS升高,SOD活力增强;与PA组比较,PH细胞凋亡 增加,ROS升高,SOD活力增强;CO组、CA组和CH组上述各指标差异无统计学意义。结论低氧环境下丙泊酚可通过氧化应 激损伤导致PC12细胞凋亡增加。
Abstract:
Objective To investigate the mechanism by which propofol exposure causes PC12 cell apoptosis under hypoxic conditions. Methods PC12 cells were exposed to room air, 35% oxygen, or 5% oxygen (hypoxia) for 24 h in the presence of either 10 μmol/L lipid emulsion or 10 μmol/L propofol. After the treatments, the cell apoptosis was measured by flow ceytometry, and the level of reactive oxygen species (ROS) and the activity of superoxide dismutase (SOD) were evaluated. Results In room air, PC12 cells treated with propofol showed increased apoptosis rate and ROS production as compared with the cells treated with the lipid emulsion; propofol treatment of the cells exposed to 35% oxygen showed obvious enhancement of the apoptosis rate, ROS production and SOD activity. Under the hypoxic condition, propofol treatment even further increased the apoptosis rate, ROS production and SOD activity. Lipid emulsion caused no such changes in cells exposed to room air, 35% oxygen or 5% oxygen. Conclusion Under hypoxic conditions, propofol can cause apoptosis in PC12 cells by inducing oxidative stress injury.

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更新日期/Last Update: 1900-01-01