[1]戚之琳,刘银华,齐世美,等.红景天苷通过抑制NOX2-ROS-MAPKs信号途径保护H2O2诱导的PC12细胞凋亡[J].南方医科大学学报,2017,(02):178.
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红景天苷通过抑制NOX2-ROS-MAPKs信号途径保护H2O2诱导的PC12细胞凋亡()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年02期
页码:
178
栏目:
出版日期:
2017-01-22

文章信息/Info

Title:
Salidroside protects PC12 cells from H2O2-induced apoptosis via suppressing NOX2-ROS-MAPKs signaling pathway
作者:
戚之琳刘银华齐世美凌烈锋冯遵永李强
关键词:
红景天苷神经保护NOX2ROSMAPKs
Keywords:
salidroside neuroprotection NOX2 reactive oxygen species mitogen-activated protein kinases
摘要:
目的探讨红景天苷保护H2O2诱导PC12细胞凋亡的分子机制。方法PC12细胞置于含10%马血清、5%胎牛血清的高糖 DMEM完全培养基中常规培养。不同浓度的红景天苷预处理细胞2 h,然后H2O2作用细胞特定的时间,Western blot检测细胞凋 亡相关蛋白PARP,caspase 3的表达及胞内信号分子p38,ERK和JNK的磷酸化水平;DAPI染色检测细胞核的形态改变;ROS检 测试剂盒检测胞内ROS的水平;膜质分离实验检测NOX2的两个重要亚基gp91phox和p47phox在胞膜和胞质中的含量;免疫共沉淀 实验检测gp91phox和p47phox的结合。结果红景天苷浓度依赖性的抑制H2O2诱导PC12细胞凋亡;减弱H2O2诱导p38,JNK和ERK 的磷酸化;减少H2O2诱发的ROS 释放;抑制gp91phox和p47phox的结合,进而抑制NOX2 的活性。结论红景天苷通过抑制 NOX2-ROS-MAPKs信号通路保护H2O2诱导PC12细胞凋亡。
Abstract:
Objective To investigate the molecular mechanism by which salidroside protects PC12 cells from H2O2-induced apoptosis. Methods PC12 cells cultured in DMEM supplemented with 10% horse serum and 5% fetal bovine serum were pretreated with different doses of salidroside for 2 h and then stimulated with H2O2 for different lengths of time. The expression levels of PARP and caspase 3 and the phosphorylation of p38, ERK and JNK were determined with Western blotting. The cell nuclear morphology was observed after DAPI staining. The production of ROS was detected using a ROS detection kit, and the levels of gp91phox and p47phox in the membrane and cytoplasm were detected by membrane-cytoplasm separation experiment; the binding between gp91phox and p47phox was assayed by coimmunoprecipitation experiment. Results Salidroside dose-dependently suppressed cell apoptosis, lowered phosphorylation levels of p38, ERK and JNK, inhibited the production of ROS, reduced the binding between gp91phox and p47phox, and inhibited the activity of NOX2 in PC12 cells exposed to H2O2. Conclusion Salidroside protects PC12 cells from H2O2-induced apoptosis at least partly by suppressing NOX2-ROS-MAPKs signaling pathway.

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[2].红景天苷通过抑制凋亡相关蛋白的表达保护缺氧对培养神经干细胞的损伤[J].南方医科大学学报,2013,(07):962.
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更新日期/Last Update: 1900-01-01