[1]张卫东,张浩,汪海,等.右美托咪定对谷氨酸所致PC12细胞损伤的保护作用及其机制[J].南方医科大学学报,2017,(02):150.
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右美托咪定对谷氨酸所致PC12细胞损伤的保护作用及其机制()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2017年02期
页码:
150
栏目:
出版日期:
2017-01-22

文章信息/Info

Title:
Protective effect of dexmedetomidine against glutamate-induced cytotoxicity in PC12 cells and its mechanism
作者:
张卫东张浩汪海张娜杜春彦余军冯泽国
关键词:
右美托咪定PC12细胞谷氨酸
Keywords:
dexmedetomidine PC12 cells glutamate
摘要:
目的研究右美托咪定(Dex)对谷氨酸所致PC12细胞损伤的保护作用及机制。方法应用高浓度谷氨酸处理PC12细胞以 建立细胞缺氧损伤模型。应用MTT法测定细胞存活率,采用试剂盒分别测定细胞培养液中乳酸脱氢酶释放量,细胞丙二醛含 量和超氧化物歧化酶活力,DCFH-DA染色流式细胞仪检测细胞内活性氧水平,Fluo-8染色流式细胞仪检测细胞内钙离子含量, JC-1染色流式细胞仪检测细胞线粒体膜电位。结果在0.01~100 μmol/L浓度范围内,Dex浓度依赖性地拮抗谷氨酸所致PC12 细胞损伤,至100 μmol/L时,细胞存活率达到正常组的(86.6±2.2)%,显著高于模型组(P<0.01),乳酸脱氢酶释放量为正常组的 1.4±0.1倍,显著低于模型组(P<0.01)。1 μmol/L Dex预处理谷氨酸损伤的PC12细胞,与模型组相比,能够显著降低丙二醛含量 (P<0.01),提高超氧化物歧化酶活力(P<0.01),抑制胞内活性氧过度生成(P<0.01),降低细胞内Ca2+浓度(P<0.01),稳定细胞线 粒体膜电位(P<0.01)。结论Dex对谷氨酸所致PC12细胞损伤具有保护作用,其机制可能与Dex抗氧化和抑制细胞内钙超载, 保护线粒体功能相关。
Abstract:
Objective To investigate the protective effects of dexmedetomidine (Dex) against glutamate-induced cytotoxicity in PC12 cells and its mechanism. Methods PC12 cells were treated with varying concentrations of dexmedetomidine 1 h before exposure to a high concentration of glutamate. The cell viability was measured by MTT assay, and LDH release, MDA content and SOD activity were measured. The level of ROS was tested by DCFH-DA staining and flow cytometry. The level of intracellular Ca2+ was detected by Fluo-8 staining and flow cytometry, and the mitochondrial membrane potential (MMP) was determined with JC-1 staining and flow cytometry. Results Within the concentration range of 0.01 to 100 μmol/L, Dex dose-dependently protected PC12 cells against glutamate-induced cytotoxicity. Treatment with 100 μmol/L Dex significantly increased the cell viability to (86.6±2.2)% of that of the control cells (P<0.01) and decreased LDH release to 1.4±0.1 folds of the control level (P<0.01). In PC12 cells exposed to glutamate, Dex pretreatment significantly reduced MDA content (P<0.01), enhanced SOD activity (P<0.01), inhibited ROS overproduction (P<0.01), reduced intracellular Ca2 + level (P<0.01) and maintained a stable MMP (P<0.01). Conclusion Dexmedetomidine can protect PC12 cells against glutamate-induced injury possibly in relation with its anti-oxidative activity, inhibitory effect on intracellular calcium overload and protective effect of the mitochondria.

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更新日期/Last Update: 1900-01-01