[1]林水苗,夏琼,张余琴,等.MiR-124通过靶向PRRX1调节结直肠癌细胞的辐射敏感性[J].南方医科大学学报,2016,(08):1110.
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MiR-124通过靶向PRRX1调节结直肠癌细胞的辐射敏感性()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2016年08期
页码:
1110
栏目:
出版日期:
2016-08-05

文章信息/Info

Title:
miR-124 regulates radiosensitivity of colorectal cancer cells by targeting PRRX1
作者:
林水苗夏琼张余琴孙爱民石玉生郑林陈龙华
关键词:
结直肠癌miR-124放射敏感性PRRX1
Keywords:
colorectalcancer miR-124 radiosensitivity PRRX1
摘要:
目的检测结直肠癌细胞及组织标本中miR-124的表达,探讨其与辐射敏感性的关系。方法qRT-PCR检测结直肠癌细胞 株和组织标本中miR-124的表达,建立过表达和干扰miR-124细胞株,功能实验探讨其对结直肠癌细胞辐射敏感性的影响;生 物信息学预测miR-124下游调控的靶基因,双荧光素酶报告系统进一步验证。结果miR-124在结直肠癌细胞株和组织标本中 表达下调;过表达miR-124 增强结直肠细胞辐射敏感性,反之,抑制miR-124 后细胞辐射敏感性降低;miR-124 通过直接抑制 PRRX1调节结直肠细胞辐射敏感性。结论miR-124可以通过直接靶向PRRX1增加结直肠癌细胞的辐射敏感性,为提高临床 结肠癌辐射敏感性提供一个靶点。
Abstract:
Objective To detect the expression of miR-124 in colorectal carcinoma (CRC) cells and tissue specimens and analyze its association with the radiosensitivity of the cells. Methods The expression of miR-124 in CRC cell lines and tissues were detected using qRT-PCR. The effect of miR-124 in modulating cell radiosensitivity was assessed in CRC cells with miRNA-124 overexpression and miRNA-124 knockdown, and bioinformatics prediction and dual luciferase reporter system were employed to identify the direct target of miR-124. Results miR-124 expression was down-regulated in CRC cell lines and tissues. CRC cells over-expressing miR-124 showed an obviously enhanced radiosensitivity, whereas miR-124 knockdown resulted in a reduced radiosensitivity of the cells. Bioinformatics prediction and dual luciferase reporter system verified PRRX1 as a direct target of miR-124, which regulated the radiosensitivity of CRC cells by directly inhibiting PRRX1. Conclusion miR-124 can enhance the radiosensitivity of CRC cells by directly targeting PRRX1, which provides a target for improving the therapeutic effect of radiotherapy of CRC.

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更新日期/Last Update: 1900-01-01