[1]林少雄,张永,鲁娟,等.干扰内皮素-1 可抑制鼻咽癌细胞增殖、迁移和侵袭[J].南方医科大学学报,2016,(07):915.
点击复制

干扰内皮素-1 可抑制鼻咽癌细胞增殖、迁移和侵袭()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2016年07期
页码:
915
栏目:
出版日期:
2016-07-15

文章信息/Info

Title:
Silencing of endothelin-1 suppresses growth, migration, and invasion of nasopharyngeal carcinoma cells in vitro
作者:
林少雄张永鲁娟刘雄李湘平
关键词:
内皮素-1鼻咽癌迁移侵袭
Keywords:
endothelin-1 nasopharyngeal carcinoma migration invasion
摘要:
目的探讨干扰内皮素-1(ET-1)基因对鼻咽癌细胞增殖、迁移及侵袭的影响以及相应的分子机制。方法构建ET-1 shRNA慢病毒干扰载体,成功包装慢病毒后感染鼻咽癌5-8F细胞。Western blotting检测ET-1干扰效率。利用MTT、细胞周期 检测、平板克隆形成实验、Transwell 小室、Boyden 小室以及裸鼠皮下成瘤实验分别检测细胞增殖、迁移、侵袭能力的改变。 Western blotting 检测上皮间质转化(EMT)相应基因表达改变。结果与对照组细胞相比,鼻咽癌细胞5-8F 感染慢病毒 shRNA-ET-1后ET-1的表达水平显著降低。MTT实验结果显示,在抑制ET-1表达后细胞增殖能力显著下降。流式细胞仪检测 细胞周期显示干扰ET-1表达,G1细胞数明显增多。平板克隆形成实验结果显示干扰ET-1表达,癌细胞克隆形成能力显著下 降。皮下裸鼠成瘤实验结果同样显示,ET-1表达抑制后癌细胞成瘤能力显著下降。Transwell和Boyden小室结果表明,ET-1表 达沉默后可显著降低细胞的迁移和侵袭能力。通过检测ET-1 沉默后EMT 相关分子的表达改变,结果显示上皮标志物 E-cadherin和CK18表达增高,而间质标志物Vimentin和N-cadherin表达降低。结论ET-1在鼻咽癌中可通过调控上皮间质转 化相关基因促进细胞增殖、迁移和侵袭能力。
Abstract:
Objective To explore the role of endothelin-1 (ET-1) gene in regulating the proliferation, migration and invasion of nasopharyngeal carcinoma cells. Methods A lentivirus-mediated shRNA-ET-1 vector was infected into 5-8F cells, and the interference efficiency was examined with Western blotting. MTT assay, cell cycle analysis, plate colony formation assay, Transwell assay, Boyden chamber assay and tumor growth assay were carried out to analyze the changes in cell proliferation, migration and invasion. The expressions of genes related with epithelial-mesenchymal transition (EMT) were examined using Western blotting. Results shRNA-ET-1 transfection significantly inhibited the expression of ET-1, and suppressed the growth, migration and invasion of 5-8F cells. ET-1 knockdown enhanced the expression of E-cadherin and CK18 and inhibited the expression of N-cadherin and vimentin. Conclusion ET-1 promotes cell growth, migration and invasion by modulating the genes associated with epithelial-mesenchymal transition in nasopharyngeal carcinoma cells.

相似文献/References:

[1]陈智贤,孙爱民,陈勇,等.放射敏感性、X线辐射剂量对鼻咽癌细胞miR-7表达的影响[J].南方医科大学学报,2010,(08):1810.
 CHEN Zhi-xian,SUN Ai-min,CHEN Yong,et al.Effects of radiosensitivity and X-ray dose on miR-7 expression in nasopharyngeal carcinoma[J].,2010,(07):1810.
[2]熊艺颖,黄仲曦.来自不同地区的两组鼻咽癌活检组织样本共同失调的基因功能及通路[J].南方医科大学学报,2011,(03):414.
[3]刘晓东,季明芳.p53通路相关基因和EZH2在鼻咽癌中的表达关系及意义[J].南方医科大学学报,2011,(06):1029.
[4]江庆萍,刘少颜,何秀芳,等.鼻咽癌中MAP3K5和Epstein-Barr病毒编码的miR-BART22表达的关系及意义[J].南方医科大学学报,2011,(07):1146.
[5]柏志全,李华荣,张海峰,等.氯通道在藤黄酸诱导低分化鼻咽癌细胞凋亡中的作用[J].南方医科大学学报,2011,(08):1304.
[6]陈静平,龙军,张宏征.EB病毒壳抗原抗体定性检测在鼻咽癌筛查中的作用[J].南方医科大学学报,2011,(09):1637.
[7]王宏梅,石玉生,李启生,等.毛细管扩张症突变基因启动子区单核苷酸多态性位点rs189037与广东鼻咽癌相关性研究[J].南方医科大学学报,2011,(11):1863.
[8]李贵平,黄凯,张辉,等.188Re-Herceptin放免导向治疗鼻咽癌裸鼠模型的实验研究[J].南方医科大学学报,2006,(04):459.
 LI Gui-ping,HUANG Kai,ZHANG Hui Departmant of Nuclear Medicine,et al.Efficacy of 188Re-Herceptin radioimmunotherapy in nude mouse model of nasopharyngeal carcinoma[J].,2006,(07):459.
[9]韩春,王爽,刘莉,等.鼻咽癌细胞肝转移亚株的建立[J].南方医科大学学报,2006,(05):654.
 HAN Chun,WANG Shuang,LIU Li,et al.Establishment of hepatic metastatic subline of nasopharyngeal carcinoma cell line[J].,2006,(07):654.
[10]王剑,吴德华,陈龙华.铜绿假单胞菌制剂抑制鼻咽癌细胞体外增殖[J].南方医科大学学报,2012,(04):544.

更新日期/Last Update: 1900-01-01