[1]严鑫,钟锐华,刘锦红,等.HBV转基因小鼠体内淋巴细胞表型特点及干扰素α对其的影响[J].南方医科大学学报,2016,(06):870.
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HBV转基因小鼠体内淋巴细胞表型特点及干扰素α对其的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2016年06期
页码:
870
栏目:
出版日期:
2016-06-20

文章信息/Info

Title:
Characteristics of lymphocyte phenotypes in HBV transgenic mice and the effect of interferon-α: a preliminary study
作者:
严鑫钟锐华刘锦红周扬唐利波李咏茵刘光泽侯金林
关键词:
乙型肝炎病毒转基因小鼠干扰素α免疫调节
Keywords:
hepatitis B virus transgenic mouse interferon alpha immunoregulation
摘要:
目的探讨乙型肝炎病毒(HBV)转基因小鼠体内淋巴细胞表型特点及干扰素α对其病毒学指标和免疫细胞表型的影响。 方法选取HBV 转基因小鼠和野生型(WT)小鼠,采用ELISA检测HBV转基因小鼠血清HBsAg和HBcAb水平及两组小鼠血 清IL-21及IL-6水平,分离肝、脾以及外周血淋巴细胞,流式细胞术检测CD4+T和CD19+B细胞频数;给予9只HBV转基因小鼠 重组鼠源性干扰素α(rmIFN-α)皮下注射,同时9只HBV转基因小鼠相应注射PBS,观察其血清HBsAg、HBV DNA、IL-6、IL-21 水平的变化以及外周血CD4+T和CD19+B细胞频数变化。结果HBV转基因小鼠的血清HBsAg水平较高并能检测到HBcAb, 其血清IL-21及IL-6水平较WT小鼠显著升高(P<0.05);HBV转基因小鼠外周血、肝脏以及脾脏淋巴细胞中CD4+T细胞频数较 WT小鼠均明显低下(P<0.05),但其肝脏CD19+B细胞频数明显高于WT小鼠(P<0.05);HBV转基因小鼠肝内CD4+T细胞频数 与血清HBsAg水平呈负相关,而肝内CD19+B细胞频数与血清HBcAb水平呈正相关;rmIFN-α处理可显著提高HBV 转基因小 鼠外周血CD4+T和CD19+B细胞频数以及血清IL-6水平(P<0.05)。结论HBV转基因小鼠体内淋巴细胞亚群频数异常,外源性 干扰素α可通过调节淋巴细胞亚群频数发挥免疫调节作用。
Abstract:
Objective To analyze the characteristics of lymphocyte phenotypes in hepatitis B virus (HBV) transgenic mice and the effect of exogenous interferon-α on virological profiles and lymphocytes phenotypes of the mice. Methods HBV transgenic mice and wild-type (WT) mice were examined for serum levels of HBsAg, HBcAb, IL-21, and IL-6 using ELISA. The frequencies of CD4+T and CD19+B cells separated from the liver, spleen, and peripheral blood were detected by flow cytometry. Nine HBV transgenic mice were injected subcutaneously with recombinant mouse interferon alpha (rmIFN-α) and another 9 transgenic mice were injected with PBS, and their HBsAg, HBV DNA, IL-6, and IL-21 levels and frequencies of peripheral blood CD4+T and CD19+B cells were detected. Results HBV transgenic mice showed a high level of HBsAg with a detectable level of HBcAb and significantly increased serum levels of IL-21 and IL-6 as compared with WT mice (P<0.05). The transgenic mice had a significantly lower frequency of CD4+ T cells in the peripheral blood, liver and spleen (P<0.05) but a significantly higher frequency of CD19+ B cells in the liver (P<0.05). An inverse correlation between intrahepatic CD4+ T cell frequency and serum HBsAg level while a positive correlation between intrahepatic CD19+ B cell frequency and HBcAb level were found in HBV transgenic mice. Administration of rmIFN-α significantly increased the frequencies of CD4+ T and CD19+ B cells in the peripheral blood and the serum level of IL-6 in HBV transgenic mice (P<0.05). Conclusion HBV transgenic mice have lymphocyte subset dysregulation and exogenous interferon-α can modulate the immune function of the mice by regulating the frequencies of lymphocyte subsets.

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更新日期/Last Update: 1900-01-01