[1]李理,覃永保,马刚,等.氯化锂复合磷酸钙骨水泥可促进大鼠胫骨骨缺损修复[J].南方医科大学学报,2016,(06):824.
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氯化锂复合磷酸钙骨水泥可促进大鼠胫骨骨缺损修复()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2016年06期
页码:
824
栏目:
出版日期:
2016-06-20

文章信息/Info

Title:
Effect of a lithium-doped calcium phosphate cement in promoting tibial bone defect repair in rats
作者:
李理覃永保马刚李兵
关键词:
磷酸钙骨水泥氯化锂显微CTHE染色骨缺损修复
Keywords:
calcium phosphate cement lithium chloride micro-CT bone defects repair
摘要:
目的观察磷酸钙骨水泥(CPC)与氯化锂复合磷酸钙骨水泥(Li/CPC)对SD大鼠骨折愈合的影响。方法取20只6月龄雌 性SD大鼠,设立Li/CPC实验组(n=10)及CPC对照组(n=10),分别行双侧胫骨骨缺损填充术,术后1、2月每组分别处死5只大 鼠,取胫骨标本行显微CT(Micro-CT)检测及组织学切片HE染色,进行比较分析。结果Micro-CT扫描显示,Li/CPC组骨缺损 修复效果比CPC组好,Li/CPC组材料周围新骨体积分数(BV/TV)较CPC组高,骨小梁分离度(Tb.Sp)较小,差异有统计学意义 (P<0.05);组织学HE染色显示,Li/CPC组纤维骨痂及骨样骨痂出现较早,且数量较多,骨愈合质量明显高于CPC组。结论Li/ CPC具有较好的骨传导性能,能明显促进骨缺损修复。
Abstract:
Objective To evaluate the effects a lithium chloride-doped calcium phosphate cement (Li/CPC) on promoting tibial defect repair in rats. Methods Twenty 6-month-old female SD rats randomized into Li/CPC (n=10) and CPC control (n=10) groups. Surgery was performed to create bone defects at the bilateral tibia, which were filled with either of the cement. Five rats from each group were sacrificed at 1 and 2 months after the surgery for micro-CT examination and HE staining of the tibia. Results Micro-CT showed better repair of bone defects in Li/CPC group, which had a significantly higher new bone volume fraction (BV/TV) and a lower trabecular separation/spacing (Tb.Sp) than the control group (P<0.05). HE staining showed an earlier appearance of fiber and osteoid callus in Li/CPC group than CPC control group. The number and quality of bone healing was significantly higher in Li/CPC group than in CPC group. Conclusion Li/CPC possessed better osteoinductivity and can significantly promote bone defect repair in rats.

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更新日期/Last Update: 1900-01-01