[1]乐珍,沈君菁,黄启涛,等.miR-135b通过靶向FOXO1促进子宫内膜癌细胞的增殖[J].南方医科大学学报,2016,(05):675.
点击复制

miR-135b通过靶向FOXO1促进子宫内膜癌细胞的增殖()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2016年05期
页码:
675
栏目:
出版日期:
2016-05-15

文章信息/Info

Title:
MiR-135b promotes proliferation of endometrial carcinoma cells by targeting FOXO1
作者:
乐珍沈君菁黄启涛秦逸飞李学农刘国炳
关键词:
子宫内膜癌增殖FOXO1 miR-135b
Keywords:
endometrial carcinoma proliferation FOXO1 miR-135b
摘要:
目的探讨miR-135b在子宫内膜癌中的表达及对子宫内膜癌细胞增殖的作用的机制。方法运用RT-PCR的方法检测22 对子宫内膜癌组织及相应的癌旁组织中的miR-135b 和FOXO1 的表达;运用RT-PCR 的方法检测JEC、Ishikawa、HEC-1-B、 RL-952这4种子宫内膜癌细胞株中miR-135b和FOXO1的表达。分别转染miR-135b的模拟物抑制物于子宫内膜癌细胞株,通 过RT-PCR检测转染的效果,通过MTT增殖试验检测对子宫内膜癌细胞增殖的影响。通过RT-PCR的方法检测FOXO1 mRNA 的变化,Western blotting 检测在FOXO1 蛋白的变化。结果miR-135b 在子宫内膜癌组织中较对应的癌旁组织表达增高(P< 0.05), FOXO1在子宫内膜癌组织中较对应的癌旁组织表达降低(P<0.05)。荧光定量PCR显示子宫内膜癌细胞中miR-135b与 FOXO1表达趋势成负相关。miR-135b能促进子宫内膜癌细胞增殖(P<0.05)。上调miR-135b能够降低FOXO1 mRNA和蛋白 的表达(P<0.05),下调miR-135b能够提高FOXO1 mRNA和蛋白的表达(P<0.05)。结论miR-135b在子宫内膜癌的发生发展 中发挥重要的作用,其部分分子机制可能是通过调控靶基因FOXO1而发挥作用。
Abstract:
Objective To explore the expression of miR-135b in endometrial carcinoma and the mechanism by which miR-135b promotes the proliferation of endometrial cancer cells. Methods The expressions of miR-135b and FOXO1 were using RT-PCR detected in 22 fresh endometrial cancer tissues and paired adjacent tissues and also in endometrial cancer cell lines JEC, Ishikawa, HEC-1-B, and RL-952. The RL-952 and Ishikawa cell lines were transfected with miR-135b mimics or inhibitors, and the changes in their proliferative activity were detected with MTT assay; the expressions of FOXO1 mRNA and protein were detected by RT-PCR and Western blotting, respectively. Results The expression of miRNA135b was significantly up-regulated and FOXO1 expression was down-regulated in endometrial carcinoma tissues as compared with the adjacent tissues (P<0.05). The mRNA expression of miR-135b was negatively correlated with the expression of FOXO1 in endometrial carcinoma. In RL-952 and Ishikawa cell lines, transfection with miR-135b mimics obviously promoted the cell proliferation (P<0.05). Up-regulation of miR-135b significantly decreased the expressions of FOXO1 protein and mRNA (P<0.05), and downregulation of miR-135b increased FOXO1 expressions (P<0.05). Conclusions MiR-135b plays an important role in the occurrence and development of endometrial carcinoma partially by regulating its target gene FOXO1.

相似文献/References:

[1]许茹,夏文杰,戎霞,等.人血小板裂解液替代胎牛血清促进骨髓间质干细胞的增殖[J].南方医科大学学报,2011,(08):1396.
[2]贾建军,王自能,刘革修,等.肿瘤浸润性树突状细胞在子宫内膜癌中的凋亡及其Fas、FasL表达意义[J].南方医科大学学报,2011,(10):1693.
[3]张俊,谢珊珊,韩晓霞,等.血管内皮生长因子对骨髓源间充质干细胞增殖的影响及其信号机制[J].南方医科大学学报,2011,(10):1697.
[4]孙凯,王伟,雷尚通,等.MicroRNA-221通过抑制CDKN1C/p57 表达促进结肠癌细胞增殖[J].南方医科大学学报,2011,(11):1885.
[5]王欢,肖松舒,曾飞.子宫内膜癌合并输卵管癌1例报告[J].南方医科大学学报,2011,(12):2093.
[6]刁冬梅,宋永春,侯妮,等.神经生长因子对不同胰腺癌细胞的刺激作用及其与细胞Trk-A表达的关系[J].南方医科大学学报,2012,(03):296.
[7]韦安阳,刘洋,何书华,等.糖尿病性大鼠阴茎海绵体平滑肌细胞凋亡和增殖特征分析[J].南方医科大学学报,2012,(02):155.
[8]全天一,何本夫,刘铁坚,等.着丝粒蛋白-H对人胃癌细胞增殖的影响[J].南方医科大学学报,2012,(02):265.
[9]张瑜,张怡,林秋华,等.孕激素对人子宫内膜癌细胞系Ishikawa影响的蛋白质组学研究[J].南方医科大学学报,2006,(08):1110.
 ZHANG Yu,ZHANG Yi,LIN Qiu-hua Department of Obstetrics and Gynecology,et al.Progesterone-modulated proteins in human endometrial cancer cell line Ishikawa[J].,2006,(05):1110.
[10]王剑,吴德华,陈龙华.铜绿假单胞菌制剂抑制鼻咽癌细胞体外增殖[J].南方医科大学学报,2012,(04):544.

更新日期/Last Update: 1900-01-01