[1]徐小艳,刘红伟,姜黄,等.联合检测细胞蜡块中的napsin A和甲状腺转录因子-1有助于肺腺癌胸水的诊断[J].南方医科大学学报,2015,(11):1610.
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联合检测细胞蜡块中的napsin A和甲状腺转录因子-1有助于肺腺癌胸水的诊断()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2015年11期
页码:
1610
栏目:
出版日期:
2015-11-15

文章信息/Info

Title:
Combined detection of thyroid transcription factor 1 and napsin A in pleural fluid cell
blocks facilitates cytopathologic diagnosis of pulmonary adenocarcinoma
作者:
徐小艳刘红伟姜黄李川袁淑慧杨金花
关键词:
肺腺癌细胞蜡块甲状腺转录因子-1napsin A
Keywords:
pulmonary adenocarcinoma cell blocks thyroid transcription factor 1 napsin A
摘要:
目的探讨细胞蜡块免疫组织化学napsin A和甲状腺转录因子-1(TTF-1)的检测对肺腺癌胸水的诊断价值。方法收集48
例有肺腺癌病史胸水的细胞涂片、细胞蜡块切片及对应的免疫组织化学切片,比较细胞涂片和细胞蜡块免疫组化两种方法诊断
肺腺癌阳性率;对免疫指标napsin A和TTF-1诊断肺腺癌价值进行评估。结果细胞蜡块免疫组化诊断肺腺癌阳性率高于细胞
涂片,差异具有统计学意义(84.44% vs 55.56% P<0.05)。TTF-1和napsin A在肺腺癌细胞蜡块中表达率分别为94.74%(36/38)
和78.95%(30/38),二者在间皮反应增生中均不表达,差异均具有统计学意义(P<0.05);联合napsin A和TTF-1检测有较高的诊
断价值,其诊断肺腺癌的灵敏度和特异度为97.37%和100%。结论细胞蜡块免疫组织化学napsin A和TTF-1检测较传统细胞
涂片有助于提高肺腺癌胸水诊断阳性率,二者联合检测对肺腺癌胸水有较高的诊断价值。
Abstract:
Objective To investigate the value of detecting thyroid transcription factor 1 (TTF-1) and Noval aspartic proteinase of
pepsin family A (napsin A) in pleural fluid cell blocks in cytopathologic diagnosis of pulmonary adenocarcinoma. Methods
Conventional cell smears of pleural effusions were obtained from 48 patients with a history of lung adenocarcinoma for
cytological analysis. The cell blocks were prepared using the cytological specimens and examined with immunohistochemistry
for TTF-1 and napsin A. The rates of a positive diagnosis of pulmonary adenocarcinoma were compared between the two
methods, and the diagnositic value of TTF-1 and napsin A in pleural fluid cell blocks was evaluated. Results Immunohistochemistry
of the cell block sections yielded a significantly higher positive rate of diagnosis than cytological analysis of
conventional cell smear (84.44% vs 55.56%, P<0.05). Most of the pleural fluid cell blocks showed positive expressions of TTF-1
(36/38, 94.74%) and napsin A (30/38, 78.95%), and none of samples showed TTF-1 or napsin A expression in the mesothelial
cells (P<0.05). The combination detection of TTF-1 and napsin A in pleural fluid cell blocks had a high diagnosis value with a
diagnostic sensitivity of 97.37% and a specificity of 100% for pulmonary adenocarcinoma. Conclusions The combined
detection of TTF-1 and napsin Ain pleural fluid cell blocks facilitates cytopathologic diagnosis of pulmonary adenocarcinoma.

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更新日期/Last Update: 1900-01-01