[1]万磊,刘健,黄传兵,等.雷公藤甲素对佐剂关节炎肺功能降低大鼠Notch通路受体和配体基因表达的影响[J].南方医科大学学报,2015,(10):1390.
点击复制

雷公藤甲素对佐剂关节炎肺功能降低大鼠Notch通路受体和配体基因表达的影响()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2015年10期
页码:
1390
栏目:
出版日期:
2015-10-20

文章信息/Info

Title:
Effect of triptolide on expressions of Notch receptors and ligands in rats with adjuvantinduced
arthritis and reduced pulmonary function
作者:
万磊刘健黄传兵谌曦汪元张皖东刘磊程园园冯云霞
关键词:
佐剂关节炎肺功能Notch信号通路雷公藤多甲素
Keywords:
adjuvant-induced arthritis pulmonary function Notch pathway triptolide
摘要:
目的观察雷公藤甲素(TPT)对佐剂关节炎(AA)大鼠Notch受体、配体表达的影响。方法将40只大鼠随机分为正常对照(NC)
组、模型(MC)组、甲氨喋呤(MTX)组、TPT组,每组10只,分别向除NC组外的其余动物右后足跖皮内注射弗氏完全佐剂0.1 mL
致炎,复制AA大鼠模型,致炎后第12填开始给药。NC组及MC组均给予生理盐水,其余组分别给予MTX、TPT。给药30 d后,检
测各组足趾肿胀度(E)、关节炎指数(AI)、肺功能、Notch受体/配体的变化。结果MC组大鼠E、AI、Notch3、Notch4、Delta1表达明
显升高;肺功能参数、Notch1、Jagged1、Jagged2表达降低(P<0.01);与MC组比较,TPT组肺功能参数、Notch1、Jagged1、Jagged2的
表达升高,E、AI及Notch3、Notch4、Delta1表达降低,疗效优于对照组MTX(P<0.05,P<0.01)。结论TPT可能是通过上调Delta1、
Notch3、Notch4的表达、下调Jagged1、Jagged2、Notch1表达,降低炎症反应和免疫复合物沉积,改善AA关节和肺部症状。
Abstract:
Objective To investigate the effects of triptolide on Notch receptor and ligand expressions in rats with
adjuvant-induced arthritis (AA). Methods Forty rats were randomly divided into normal control (NC) group, model (MC)
group, methotrexate group and triptolide groups. Rat models of AA were established by an intradermal injection of 0.1 mL
Freund’s complete adjuvant into the right paw. Twelve days after the injection, the rats were treated with corresponding drugs
for 30 days; the rats in NC group and MC group were given saline only. Paw edema volume (E), arthritis index (AI),
pulmonary function, histomorphologies, and Notch receptor/ ligand expression in the lung tissue were analyzed after the
treatments. Results Compared with the NC group, E, AI, Notch3, Notch4, and Delta1 expressions in the lung tissues
significantly increased while pulmonary function and pulmonary expressions of Notch1, Jagged1, and Jagged2 significantly
decreased the model rats (P<0.01). Compared with the MC group, triptolide-treated rats showed significantly improved
pulmonary functions, increased expressions of Notch1, Jagged1, and Jagged2 and decreased expressions of Notch3, Notch4,
and Delta1 in the lungs (P<0.05, P<0.01); the therapeutic effect of triptolide was better than that of methotrexate. Conclusion
Triptolide can reduce inflammatory reaction and immune complex deposition to improve joint and pulmonary symptoms in
rats with AA possibly by up-regulating the expressions of Notch3, Notch4, and Delta1 and down-regulating the expressions of
Jagged1, Jagged2, and Notch1.

相似文献/References:

[1]吴雪郡,黄英,王莹,等.619例儿童哮喘肺功能FEV1 和PEF与小气道的关系[J].南方医科大学学报,2011,(11):1900.
[2]叶泽慧,黄英,王莹,等.屋尘螨疫苗治疗儿童哮喘2 年肺功能和激素用量的动态观察[J].南方医科大学学报,2012,(11):1632.
[3]韩建芳,李涛平,冯媛,等.睡眠呼吸暂停综合征患者的持续气道正压通气治疗压力预测[J].南方医科大学学报,2012,(12):1727.
[4]康春燕,刘长庭,李婷,等.华北城市地区成人肺通气功能预计值方程[J].南方医科大学学报,2013,(11):1590.
[5]叶泽慧,黄英.性别对肥胖哮喘患儿吸入糖皮质激素后肺功能的影响[J].南方医科大学学报,2016,(12):1723.
[6]郭远波,徐金东,纪雪霞,等.右美托咪定对行肺癌根治术的患者围手术期炎症及肺功能保护作用的影响[J].南方医科大学学报,2017,(12):1673.

更新日期/Last Update: 1900-01-01