[1]朱梦叶,刘娜娜,彭斯聪,等.米诺环素抑制大鼠脊髓背角胶状质神经元超极化激活电流的作用[J].南方医科大学学报,2015,(08):1155.
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米诺环素抑制大鼠脊髓背角胶状质神经元超极化激活电流的作用()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2015年08期
页码:
1155
栏目:
出版日期:
2015-07-26

文章信息/Info

Title:
Minocycline reduces hyperpolarization-activated current in rat substantia gelatinosa
neurons
作者:
朱梦叶刘娜娜彭斯聪李凌超张达颖柳涛
关键词:
米诺环素超极化激活电流脊髓背角胶状质神经元
Keywords:
minocycline hyperpolarization-activated current substantia gelatinosa
摘要:
目的研究米诺环素对大鼠脊髓背角胶状质(SG)神经元超极化激活电流(Ih)的影响。方法选取3~5周龄雄性SD大鼠,
制作离体脊髓横切片,应用全细胞膜片钳技术记录SG神经元Ih,灌流不同浓度米诺环素(1~300 μmol/L)并观察其对Ih的影响。
结果记录的SG神经元中约50%可记录到Ih,该电流被Ih阻断剂CsCl和ZD7288阻断。米诺环素可减低Ih幅值、减小Ih电流密
度,此效应呈可逆性及剂量依赖性,半数有效抑制浓度(IC50)为34 μmol/L。结论米诺环素具有抑制脊髓背角SG神经元Ih的作
用,从而降低SG神经元兴奋性,对疼痛的调控具有重要的意义。
Abstract:
Objective To investigate the effect of minocycline on hyperpolarization-activated current (Ih) in the substantia
gelatinosa (SG) neurons in rat spinal dorsal horn. Methods In vitro spinal cord transverse slices were prepared from
3-5-week-old male Sprague-Dawley rats. Using whole-cell patch clamp technique, Ih currents were recorded before and after
bath application of minocycline (1-300 μmol/L) to the SG neurons. Results Ih currents were observed in nearly 50% of the
recorded neurons, and were blocked by Ih blocker CsCl and ZD7288. Minocycline rapidly and reversibly reduced the
amplitude of Ih and decreased the current density in a concentration-dependent manner with an IC50 of 34 μmol/L. Conclusion
Minocycline suppresses the excitability of SG neurons through inhibiting the amplitude and current density of Ih and thereby
contributes to pain modulation.

相似文献/References:

[1]陶涛,秦新月,马勋泰,等.ERK1/2通路介导米诺环素促进PC12细胞氧糖剥夺后血红素加氧酶-1的表达[J].南方医科大学学报,2015,(01):117.

更新日期/Last Update: 1900-01-01