[1]李坚,张剑,董欣敏,等.槲皮素对内毒素性心肌损伤的保护作用及机制[J].南方医科大学学报,2015,(07):1068.
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槲皮素对内毒素性心肌损伤的保护作用及机制()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2015年07期
页码:
1068
栏目:
出版日期:
2015-07-15

文章信息/Info

Title:
Quercetin protects against lipopolysaccharide-induced cardiac injury in mice
作者:
李坚张剑董欣敏邓华菲阳帆
关键词:
槲皮素内毒素心肌损伤
Keywords:
quercetin endotoxin cardiac injury
摘要:
目的观察槲皮素对内毒素心肌损伤心肌的保护作用。方法C57BL/6J 小鼠随机分为阴性对照组、槲皮素对照组、内
毒素血症组以及槲皮素干预组。阴性对照组给予等体积生理盐水腹腔注射,槲皮素干预组大鼠食用含按槲皮素的鼠饲料
(100 mg/kg)。内毒素组小鼠腹腔注射LPS(10mg/kg)诱导内毒素血症模型。槲皮素干预组大鼠先食用含槲皮素(100 mg/kg)
的饲料7 d后,腹腔注射LPS(10mg/kg)。6 h后超声心动仪测定小鼠心功能;Western blot法检测小鼠心肌组织Bax、Bcl-2、NOS
和eNOS表达水平,以及血清中NO含量。同时,记录小鼠5 d生存率,并描绘生存曲线。结果槲皮素预处理后,与内毒素组相
比,小鼠心肌功能显著得到了改善;内毒素组小鼠心肌组织中iNOS蛋白水平增高,eNOS水平降低。而经槲皮素干预后,iNOS
蛋白表达量明显降低,eNOS表达水平增高;LPS处理后,Bax显著增高,Bcl-2轻微降低。槲皮素干预后,Bax降至对照组水平,
Bax/Bcl-2比值降低;内毒素组小鼠血清NO水平增高近3.5倍。槲皮素处理后,NO含量明显降低(P<0.05);内毒素组5d后小鼠
生存率为10%,而槲皮素干预组生存率达45%(P<0.05)。结论槲皮素可减轻LPS 所致的小鼠心功能不全,改善生存率。
Abstract:
Objective To evaluate the protective effect of quercetin against lipopolysaccharide (LPS)-induced cardiac injury in
mice. Methods C57BL/6J mice were randomized into 4 groups to receive intraperitoneal injection of saline (negative control) or
LPS (20 mg/kg), or fed with quercetin (100 mg/kg for 7 days) with or without subsequent LPS injection (quercetin+LPS group
and quercetin control group, respectively). Six hour after LPS injection, the mice were tested for cardiac function with an
echocardiograph, and the protein expressions of Bax, Bcl-2, iNOS, and eNOS in the myocardium were evaluated with Western
blotting; serum NO concentration was also measured. The survival of the mice within 5 days after LPS injection was recorded
to draw the survival curve. Results Quercetin pretreatment significantly improved the cardiac function of LPS-challenged
mice (P<0.05), and attenuated LPS-induced increment in myocardial iNOS expression and decrement in eNOS level. LPS
significantly increased the myocardial Bax expression and slightly decreased Bcl-2 expression; quercetin pretreatment
decreased Bax expression to the control level and significantly lowered Bax/Bcl-2 ratio as compared with the LPS group. Serum
NO level was significantly increased by nearly 2.5 folds in LPS-challenged mice, but was markedly decreased with quercetin
pretreatment (P<0.05). The 5-day survival rate of LPS-treated mice was 10% , which was increased to 45% in quercetinpretreated
mice (P<0.05). Conclusion Quercetin can alleviate LPS-induced cardiac dysfunctions in mice to increase their
survival rate following LPS challenge.

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更新日期/Last Update: 1900-01-01