[1]彭宇声,黎 倩,王梦蕾,等.碱性亮氨酸拉链转录因子ATF样蛋白在狼疮小鼠肾脏组织中的表达及意义[J].南方医科大学学报,2015,(06):816.
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碱性亮氨酸拉链转录因子ATF样蛋白在狼疮小鼠肾脏组织中的表达及意义()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2015年06期
页码:
816
栏目:
出版日期:
2015-06-15

文章信息/Info

Title:
Expression of BATF, a member of the activator protein-1 family, in renal tissues of
MRL/lpr mice
作者:
彭宇声黎 倩王梦蕾曹 灿赖 宽曾 抗
关键词:
BATF转录因子系统性红斑狼疮狼疮性肾炎
Keywords:
BATF transcription factor systemic lupus erythematosus lupus nephritis
摘要:
目的探讨碱性亮氨酸拉链转录因子ATF样蛋白(basic leucine zipper transcription factor, ATF-like, BATF)在狼疮鼠肾脏
组织中的表达及临床意义。方法以24周龄雌性MRL/lpr小鼠作为狼疮肾炎模型组,同龄C57BL/6雌性小鼠作为正常对照组。
采用Western blot检测肾脏组织BATF的蛋白表达,采用RT-PCR检测肾脏组织中BATF、RORγt及IL-17 mRNA的表达水平,同
时检测小鼠尿蛋白、血清抗ds-DNA抗体以及肾脏组织病理的表现,并对BATFmRNA及RORγt、IL-17 mRNA相关性进行分
析。结果狼疮小鼠尿蛋白、血清抗ds-DNA抗体水平均高于正常小鼠(P<0.05)。狼疮小鼠肾脏组织病理主要表现为肾小球系
膜细胞增生,肾间质大量炎细胞浸润,而正常小鼠无明显异常。狼疮小鼠肾脏组织BATF mRNA和蛋白的表达量均较正常小鼠
升高(P<0.05)。狼疮小鼠肾脏组织RORγt、IL-17 mRNA表达量增多,且BATF mRNA的表达量与RORγt 、IL-17 mRNA表达量
呈正相关(r=0.945,0.876,P均<0.05)。结论在狼疮小鼠肾脏组织中BATF mRNA和蛋白的表达均高于对照组,BATF可能通过
上调Thl7免疫反应参与狼疮性肾炎的发病。
Abstract:
Objective To investigate the expression of BATF, a member of the activator protein-1 family, in the renal tissues of
mice with lupus nephritis. Methods The renal tissues from 24-week-old female MRL/lpr mice and age- and sex-matched
C57BL/6 mice were examined for BATF protein expressions using Western blotting and for expressions of BATF, RORγt and
IL-17A mRNA using quantitative real-time PCR. The results of laboratory examinations were analyzed in relation with the
histopathology of the mice. Results The urinary protein and ds-DNA levels were significantly higher in MRL/lpr mice than in
the control mice (P<0.05). Compared to normal control mice, MRL/lpr mice showed obvious glomerular fibrosis and
inflammatory cell infiltrating with significantly increased BATF protein and mRNA expressions (P<0.05) and RORγt and IL-17
mRNA expressions in the renal tissues (P<0.05). In MRL/lpr mice, the expression of BATF mRNA was positively correlated
with the RORγt and IL-17A mRNA expressions in the renal tissues. Conclusion The renal expressions of BATF protein and
mRNA is increased in MRL/lpr mice. BATF may participate in the immunopathogenesis of lupus nephritis by enhancing Thl7
cell response.

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更新日期/Last Update: 1900-01-01