[1]黎天尊,晏 怡,刘 强,等.细胞凋亡信号调节蛋白1对大鼠脊髓损伤后GFAP、vimentin表达及后肢运动功能的影响[J].南方医科大学学报,2015,(06):795.
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细胞凋亡信号调节蛋白1对大鼠脊髓损伤后GFAP、vimentin表达及后肢运动功能的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2015年06期
页码:
795
栏目:
出版日期:
2015-06-15

文章信息/Info

Title:
Effect of suppressing apoptosis signal regulating kinase 1 on GFAP and vimentin
expression and hindlimb mobility in rats after spinal cord injury
作者:
黎天尊晏 怡刘 强夏永智
关键词:
脊髓损伤细胞凋亡信号调节蛋白1胶质纤维酸性蛋白波形蛋白
Keywords:
spinal cord injury apoptosis signal regulating kinase 1 glial fibrillary acidic protein vimentin
摘要:
目的探讨细胞凋亡信号调节蛋白1(ASK1)对大鼠脊髓损伤(SCI)后胶质瘢痕中中间丝蛋白胶质纤维酸性蛋白(GFAP)和
波形蛋白(vimentin)的表达及损伤大鼠后肢行为学和电生理变化的影响。方法采用大鼠脊髓夹伤模型,设ASK1特异性抑制
剂Thioredoxin组(Trx组)、ASK1单克隆抗体组(Anti-ASK1组)、生理盐水对照组(Model组)及假手术组(Sham组),于损伤即刻
在损伤局部分别给予Thioredoxin、ASK1单克隆抗体、生理盐水各10 μl,Sham组大鼠仅打开椎板暴露脊髓,不造成SCI。各组大
鼠定期(SCI后1、7、14、28 d)应用Western blot和免疫荧光法检测GFAP、vimentin的表达,BBB评分检测大鼠后肢行为学变化,
体感诱发电位及运动诱发电位检测电生理的变化。结果SCI后1 d各组GFAP、vimentin的表达无明显差异,7、14、28 d Trx组、
Anti-ASK1组GFAP、vimentin的表达较Model组明显下降(P<0.01);SCI后1、7、14 d各组BBB评分及体感诱发电位、运动诱发
电位无明显差异,SCI后28 d,Trx组、Anti-ASK1组BBB评分较Model组明显升高(P<0.01),体感诱发电位及运动诱发电位潜伏
期明显缩短,波峰值明显升高(P<0.01或P<0.01)。结论抑制ASK1可减弱大鼠SCI胶质瘢痕局部GFAP、vimentin的表达,并可
促进大鼠后肢运动功能的恢复和电生理的改善。
Abstract:
Objective To investigate the effect of suppressing apoptosis signal regulating kinase 1 (ASK1) on glial fibrillary
acidic protein (GFAP) and vimentin expressions at the injury site and on hindlimb mobility in rats after spinal cord injury
(SCI). Methods The rat models of SCI were established by extradural compression of the spinal cord using an aneurysm clip.
The injured rats were treated with normal saline (model group), ASK1 specific inhibitor thioredoxin (Trx group), or ASK1
monoclonal antibody (Anti-ASK1 group), and the rats receiving a sham operation underwent laminectomy without SCI. The
expression of GFAP and vimentin were detected by Western blotting and immunofluorescence assay at 1, 7, 14 and 28 days
after SCI. The motion function of the hindlimbs of the injured rats was assessed with Basso Beattie Bresnahan (BBB) scores, and
somatosensory-evoked potentials (SEP) and motor-evoked potentials (MEP) were determined to examine the
electrophysiological changes. Results At 1 day after SCI, the expressions of GFAP and vimentin showed no significant
differences among the groups; at 7, 14 and 28 days after SCI, GFAP and vimentin expressions significantly increased in Trx and
Anti-ASK1 groups compared with those in the model group (P<0.01). The BBB scores showed no significant differences among
the groups at 1, 7 and 14 days after SCI, while at 28 days, the BBB scores in Trx and Anti-ASK1 groups were significantly
higher than those in the model group (P<0.01). At 28 days after SCI, the latent period of SEP and MEP decreased and the
amplitude increased significantly in Trx and Anti-ASK1 groups compared with that in the model group (P<0.01). Conclusion
Blocking ASK1 can inhibit the expression of GFAP and vimentin in glial scars and improve the outcomes of hindlimb mobility
in rats after SCI.

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更新日期/Last Update: 1900-01-01