[1]奈文青,刘灏,王媛媛,等.差异基因表达谱分析颈动脉不稳定斑块相关基因及可能的信号通路[J].南方医科大学学报,2015,(05):738.
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差异基因表达谱分析颈动脉不稳定斑块相关基因及可能的信号通路()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2015年05期
页码:
738
栏目:
出版日期:
2015-05-15

文章信息/Info

Title:
Differential gene expression profiling for identification of potential pathogenic genes
and pathways in carotid unstable plaques
作者:
奈文青刘灏王媛媛单兰兰傅友吴洪渊丁燕陈顺枝刘正军陈婕戴萌
关键词:
不稳定斑块动脉粥样硬化表达谱差异表达基因生物信息学
Keywords:
unstable plaque atherosclerosis expression profile differential expressed genes bioinformatics
摘要:
目的探讨颈动脉不稳定斑块发生的分子机制。方法从ArrayExpress数据库下载基因表达谱数据E-MTAB-2055。该数
据包含24例颈动脉不稳定斑块和24例稳定斑块组织,从中筛选差异基因,并通过富集分析获取与不稳定斑块有关的生物学过
程和通路。同时,通过构建差异表达基因的生物网络来寻找与该疾病高度相关的风险模块,并用荧光定量PCR法检测五对不稳
定斑块和斑块旁标本,验证风险模块中部分基因的差异表达。结果不稳定斑块中发生显著性差异表达变化的基因共有439
个,其中上调基因为232个,下调基因为207个。涉及到的生物学过程和通路大部分与炎症和免疫应答有关。生物网络和模块
分析提示CXCR4、VCL和TYROBP 可能在不稳定斑块发生发展过程中发挥着重要作用。荧光定量PCR结果显示CXCR4和
TYROBP基因表达情况与芯片数据分析结果一致。结论本研究比较完整地揭示了不稳定斑块差异表达谱特征和所涉及的生
物学过程和信号通路,其中TYROBP可能是不稳定斑块发生发展进程中新的致病基因。
Abstract:
Objective To explore the molecular mechanism in the formation of unstable plaques. Methods The cDNA
microarray E-MTAB-2055 was downloaded from ArrayExpress database to screen the differentially expressed genes in 24
ruptured plaques against 24 stable plaques. Functional enrichment analysis was conducted to define the biological processes
and pathways involved in disease progression. The protein-protein interaction network was constructed to identify the risk
modules with close interactions. Five pairs of carotid specimens were used to validate 3 differentially expressed genes of the
risk modules by real-time PCR. Results A total of 439 genes showed differential expression in our analysis, including 232
up-regulated and 207 down-regulated genes according to the data filter criteria. Immune-related biological processes and
pathways were greatly enriched. The protein-protein interaction network and module analysis suggested that TYROBP, VCL
and CXCR4 might play critical roles in the development of unstable plaques, and differential expressions of CXCR4 and
TYROBP in carotid plaques were confirmed by real-time PCR. Conclusion Our study shows the differential gene expression
profile, potential biological processes and signaling pathways involved in the process of plaque rupture. TYROBP may be a
new candidate disease gene in the pathogenesis of unstable plaques.

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更新日期/Last Update: 1900-01-01