[1]王巍,孟灿,刘佳,等.急性髓系白血病CDX2和β-catenin基因表达的相关性[J].南方医科大学学报,2015,(05):728.
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急性髓系白血病CDX2和β-catenin基因表达的相关性()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2015年05期
页码:
728
栏目:
出版日期:
2015-05-15

文章信息/Info

Title:
Expressions of CDX2 and β-catenin and their correlation in acute myeloid leukemia
作者:
王巍孟灿刘佳杨志刚
关键词:
急性髓系白血病CDX2β-catenin基因表达
Keywords:
acute myeloid leukemia CDX2 β-catenin gene expression
摘要:
目的研究急性髓系白血病(AML)患者CDX2与β-catenin基因表达的相关性。方法实时定量PCR方法检测92例初诊
AML、30例非恶性血液病患者(对照)CDX2及β-catenin mRNA表达,Western blot检测其中的26例AML、8例非恶性血液病患
者CDX2、β-catenin蛋白表达。结果AML组CDX2 mRNA和蛋白表达阳性率分别为84.78%和76.92%,对照组未检测到表达
(P<0.01);β-catenin mRNA和蛋白在两组均表达,但AML组显著高于对照组(P<0.01)。CDX2、β-catenin mRNA表达均与初诊
时白细胞数、LDH水平呈正相关(P<0.01)。AML患者获得完全缓解时,CDX2 mRNA转阴、β-catenin mRNA表达显著下降,复
发时两者均增高。AML患者CDX2与β-catenin无论在mRNA和蛋白水平表达均呈显著正相关(P<0.01)。结论AML患者存
在CDX2基因过表达及Wnt/β-catenin信号通路激活,且CDX2与β-catenin表达呈显著正相关。
Abstract:
Objective To investigate the expressions of CDX2 and β-catenin and their correlation in acute myeloid leukemia
(AML). Methods Real-time PCR was used to examine the expressions of CDX2 and β-catenin mRNA in 92 de novo AML and
30 non-malignant patients (control), and Western blot was employed to detect CDX2 and β-catenin protein expressions in 26
de novo AML and 8 non-malignant patients. Results The positivity rates of CDX2 mRNA and protein expressions in AML
group were 84.78% and 76.92%, respectively, but no CDX2 mRNA or protein expression were detected in control group (P<
0.01). The expressions of β-catenin mRNA and protein were detected in all the patients in both groups but its expressions in
AML group were significantly higher (P<0.01). The expressions of CDX2 and β-catenin mRNA were significantly correlated
with WBC counts and LDH levels (P<0.01). The expression of CDX2 mRNA was not detected and the expression of β-catenin
mRNA was decreased to normal level in AML patients with complete remission, while the expressions of CDX2 and β-catenin
mRNA were increased again in those with disease relapse. There were significantly positive correlations between CDX2 and
β-catenin expressions at both mRNA and protein levels (P<0.01). Conclusion Up-regulation of CDX2 gene and activation of
Wnt/β-catenin pathway co-exist in AML patients and the expressions of CDX2 and β-catenin are positively correlated.

相似文献/References:

[1]许露露,刘晓力,杜庆锋,等.八探针荧光原位杂交技术在急性髓系白血病诊断中的应用[J].南方医科大学学报,2011,(07):1204.

更新日期/Last Update: 1900-01-01