[1]吴共发,赵海燕,何楠,等.miR-200a在结直肠癌细胞系中的表达及其对高转移细胞系Lovo的影响[J].南方医科大学学报,2015,(03):450.
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miR-200a在结直肠癌细胞系中的表达及其对高转移细胞系Lovo的影响()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2015年03期
页码:
450
栏目:
出版日期:
2015-03-20

文章信息/Info

Title:
Expression of miR-200a in colorectal carcinoma cell lines and its effect on LoVo cells
作者:
吴共发赵海燕何楠韩慧霞
关键词:
miR-200a结肠癌增殖迁移凋亡粘附
Keywords:
miR-2000a colorectal carcinnoma proliferation migration apoptosis adhesion
摘要:
目的检测结肠癌细胞系中miR-200a对高转移细胞系Lovo的影响。方法采用荧光定量PCR法检测6种结肠癌细胞系
HCT116、HT29, LS174T、SW480、SW620和Lovo中miR-200a的表达水平,在高转移细胞系Lovo中瞬时转染miR-200a mimics
使其高表达miR-200a,检测高表达miR-200a后Lovo细胞增殖、迁移、细胞同质黏附能力和凋亡等情况的变化。结果miR-200a
在6种细胞系中存在差异性表达,其中具有高转移潜能的Lovo中表达最低,成瘤但不转移的细胞系HCT116表达最高。在Lovo
细胞高表达miR-200a后,Lovo细胞的增殖和迁移能力明显减弱、细胞同质黏附能力增强且凋亡增加。结论miR-200a在结肠
癌细胞系中表达失常,其作用可能与结肠癌细胞的侵袭和转移有关,高表达miR-200a 能抑制Lovo细胞增殖和迁移、增强细胞
间黏附能力、促进凋亡,其分子机制有待进一步研究。
Abstract:
Objective To detect miR-200a expression in human colorectal carcinnoma (CRC) cell lines and explore the role of
miR-200a in regulating the biological behavior of CRC cells. Methods Real-time quantitative RT-PCR (qRT-PCR) was used to
detect miR-200a expression levels in 6 CRC cell lines (HCT116, HT29, LS174T, SW480, SW620 and LoVo). miR-200a mimics
were transiently transfected into LoVo, and the changes in cell proliferation, apoptosis, migration, and cell-cell adhesion were
assessed using CCK-8 assay, TUNEL assay, transwell migration assay, and homogenous adhesion experiment, respectively.
Results The expression of miR-200a was down-regulated in the 6 CRC cell lines, among which the highly metastatic LoVo cell
line showed the lowest expression and the tumorigenic but non-metastatic CRC cell line HCT116 had the highest expression.
Overexpression of miR-200a depressed cell proliferation and migration but promoted cell apoptosis and cell-cell adhesion in
LoVo cells. Conclusion miR-200a plays a role in regulating the invasiveness and metastasis of CRC, and overexpression of
miR-200a causes a significant reduction of cell proliferation and migration and promotes apoptosis and cell-cell adhesion in
LoVo cells.

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更新日期/Last Update: 1900-01-01