[1]吕建平,曹志恺,Lee Jin-Moo.四波长内源光信号成像在GFAP-/-Vim-/-小鼠脑梗塞周围扩散性去极化中的应用[J].南方医科大学学报,2015,(03):417.
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四波长内源光信号成像在GFAP-/-Vim-/-小鼠脑梗塞周围扩散性去极化中的应用()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2015年03期
页码:
417
栏目:
出版日期:
2015-03-20

文章信息/Info

Title:
Application of 4-wavelength optical intrinsic signal imaging in monitoring peri-infarct
depolarizations in GFAP-/-Vim-/- mice
作者:
吕建平曹志恺Lee Jin-Moo
关键词:
脑梗塞扩散性去极化内源光信号胶质纤维酸性蛋白波形蛋白
Keywords:
brain infarction spreading depolarization optical intrinsic signal glial fibrillary acidic protein vimentin
摘要:
目的观测小鼠脑梗塞周围扩散性去极化(PIDs)的内源光信号变化特征,探索胶质纤维酸性蛋白(GFAP)和波形蛋白
(Vim)基因敲除对PIDs的影响。方法以GFAP和Vim基因敲除(GFAP-/-Vim-/-)小鼠及其野生型(GFAP+/+Vim+/+)小鼠为研究对
象,线栓法制备大脑中动脉梗塞(MCAO)模型,应用四波长内源光信号(OIS)成像技术监测两组动物4 h内PIDs的发作情况。
结果OIS成像显示PIDs在空间分布上表现为连续的、红蓝相间的弧形波,由发源处向四周缓慢播散;PIDs存在5种不同空间播
散类型:喙-尾播散类型、侧方-内侧播散类型、尾-喙播散类型、对侧播散类型和内侧-侧方播散类型。GFAP-/-Vim-/-小鼠和GFAP+/+
Vim+/+小鼠PIDs的发作次数、播散类型、速度及时程组间比较无统计学差异(P>0.05)。结论四波长OIS成像技术可获得PIDs
的高分辨率彩色影像,为更深入研究PIDs的时空特性创造了条件;GFAP和Vim基因敲除对MCAO模型制备后4 h内PIDs的发
作无显著影响。
Abstract:
Objective To study optical intrinsic signal (OIS) imaging of peri-infarct depolarizations (PIDs) in mice and
investigate the influence of knockout of glial fibrillary acidic protein and vimentin on PIDs. Methods GFAP-/-Vim-/- mice and
GFAP+/+Vim+/+ mice were subjected to MCAO by standard intraluminal filament method. The main characteristics of PIDs in 4 h
were studied by 4-wavelength OIS imaging technique. Results PIDs were identified as consistent, red and blue interaction
waves in the cortical reflectance that slowly propagated peripherally from the origin site. There were 5 patterns of PID
propagation, namely rostro-caudal, latero-medial, caudo-rostral, contralateral and medial-lateral. No significant differences
were found in PID frequency, propagation patterns, velocity or duration time between the two groups (P>0.05). Conclusion
The 4-wavelength OIS system allows acquisition of high temporal-spatial resolution color images for analyzing
temporal-spatial characteristics of PIDs in detail. Knockout of GFAP and vimentin do not affect PIDs in 4 h following middle
cerebral artery occlusion.

相似文献/References:

[1]冯长征,李扬彬,谭理连,等.缺血性脑梗塞CT表现与梗塞后神经细胞凋亡及坏死的相关性[J].南方医科大学学报,2005,(04):444.
 FENG Chang-zheng,LI Yang-bin,TAN Li-lian,et al.CT findings of ischemic infarction in relation to neuronal damage in rabbits[J].,2005,(03):444.

更新日期/Last Update: 1900-01-01