[1]徐亚文,陈玢屾,许凯,等.RNA干扰IgG表达对人前列腺癌细胞株PC3放射敏感性的影响[J].南方医科大学学报,2015,(03):397.
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RNA干扰IgG表达对人前列腺癌细胞株PC3放射敏感性的影响()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2015年03期
页码:
397
栏目:
出版日期:
2015-03-20

文章信息/Info

Title:
Effect of IgG gene silencing by RNA interference on radiosensitivity of prostate cancer
PC3 cells
作者:
徐亚文陈玢屾许凯李炳坤温勇赵朋朋
关键词:
免疫球蛋白GsiRNA前列腺癌放射敏感性
Keywords:
IgG RNA interference prostate cancer radiosensitivity
摘要:
目的探讨RNA干扰沉默IgG 表达对人前列腺癌PC3 细胞株放射敏感性的影响。方法将IgG FC 段受体RNA质粒
(FCGR1AshRNA)和阴性对照质粒(NCshRNA)转染人前列腺癌PC3细胞,Q-PCR和Western-blot检测IgG表达。以60Co γ射线
0、2、4、6、8、10 Gy分别照射空白对照组、NCshRNA组、FCGR1AshRNA组细胞;照射48 h后,MTS法检测细胞增殖状态;照射
12、24、48 h后,流式细胞术检测细胞凋亡率。重点探讨6Gy射线照射后不同时间的敏感性、增值率、抑制率与凋亡率。结果质
粒转染前列腺癌PC3细胞后,FCGR1AshRNA与NCshRNA组和空白对照组相比,IgG表达水平明显下降,细胞增殖受抑制(P<
0.05)。不同放射剂量下48 h后及6Gy射线照射后的不同时间段,FCGR1AshRNA组的增值率减低,凋亡率升高(P<0.05)。结
论RNA干扰抑制IgG表达能提高PC3细胞对放射线的敏感性,IgG基因可能是联合放疗治疗前列腺癌的理想靶点。
Abstract:
Objective To investigate the effect of RNA interference of IgG gene on the radiosensitivity of the human prostate
cancer PC3 cell line. Methods PC3 cells were trasnfected via lipofectamine by the shRNA vector FCGR1AshRNA targeting the
Fc segment of IgG, using NCshRNA as the negative control. Q-PCR and Western blotting were used to analyze the expression
of IgG in the trasnfected cells. The cells were then exposed to 60Co γ ray at 0, 2, 4, 6, 8, 10 Gy, and the cell proliferation was
evaluated by MTS and the cells apoptosis estimated by flow cytometry at 12, 24 and 48 h. Results MTS assay showed that 60Co
γ ray significantly inhibited the proliferation of PC3 cells transfected with FCGR1AshRNA as compared with
NCshRNA-transfected and blank control cells (P<0.05). Flow cytometry showed that the cell apoptosis rate was significantly
higher in FCGR1AshRNA group than in NCshRNA and blank control groups at 48 h after γ ray exposure (P<0.05). At 12, 24
and 48 h after 6 Gy radiation, the cells in FCGR1AshRNA group showed a significantly lowered proliferation rate and an
increased apoptosis rate (P<0.05). Conclusion The shRNA targeting IgG gene can significantly enhance the sensitivity of PC3
cells to radiation. The combination of RNA interference targeting IgG gene with radiotherapy may be more effective in the
treatment of prostate cancer.

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更新日期/Last Update: 1900-01-01